Abstract Intra-specific chemical variability in the natural population of Gloriosa superba from Eastern Ghats (India) was recorded through validated HPTLC method and in vitro antiarthritic activity of elite chemotypes was evaluated. In total, 36 population(s) of G. superba were mapped and, collected from the phytogeography and densitometric quantification through validated HPTLC-UV method revealed a significant (p > 0.05) difference in the metabolite content of germplasm(s). Colchicine and gloriosine content varied from 0.005 to 0.275% and 0.003 to 0.119%, maximum was recorded in NBG-34 (Tirupati, Andhra Pradesh) and NBG-70 (Panchgani hill, Maharashtra) respectively. On cluster analysis, six chemotypes were selected for in vitro antiarthritic activity, although activity was not significantly different. The elite chemotype with highest metabolite(s) content did not exhibit the highest biological activity and thus suggests the synergistic action of extract toward the pharmacological activity. The study promotes the use of G. superba in herbal formulation development as an adjuvant therapy in gouty arthritis and will aid in site-specific exploration of elite chemotype(s) with validated pharmacological action to meet out the medicinal and commercial demands. GRAPHICAL ABSTRACT

中文翻译：

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同时 HPTLC-UV 定量分析从印度东高止山脉收集的 Gloriosa superba L. 自然种群中秋水仙碱和 Gloriosine 生物碱，用于鉴定优良化学型

摘要 通过有效的 HPTLC 方法记录了来自东高止山脉（印度）的 Gloriosa superba 自然种群的特异性化学变异性，并评估了优良化学型的体外抗关节炎活性。总共绘制了 36 个 G. superba 种群，并通过经过验证的 HPTLC-UV 方法从植物地理学和光密度定量中收集，揭示了种质代谢物含量的显着（p > 0.05）差异。秋水仙碱和Gloriosine 含量从0.005 到0.275% 和0.003 到0.119% 不等，最大值分别记录在NBG-34（蒂鲁帕蒂，安得拉邦）和NBG-70（Panchgani hill，马哈拉施特拉邦）。在聚类分析中，选择了六种化学型的体外抗关节炎活性，尽管活性没有显着差异。具有最高代谢物含量的优良化学型没有表现出最高的生物活性，因此表明提取物对药理活性的协同作用。该研究促进了 G. superba 在草药配方开发中作为痛风性关节炎的辅助疗法的使用，并将有助于对具有经过验证的药理作用的精英化学型进行特定部位的探索，以满足医药和商业需求。图形概要 superba 在草药配方开发中作为痛风性关节炎的辅助疗法，将有助于对具有经过验证的药理作用的精英化学型进行特定部位的探索，以满足医药和商业需求。图形概要 superba 在草药制剂开发中作为痛风性关节炎的辅助疗法，将有助于对具有经过验证的药理作用的精英化学型进行特定部位的探索，以满足医药和商业需求。图形概要