Abstract Rhodiola rosea is a well-known adaptogen plant with antioxidant, antidepressant, anti-stress, and anti-dementive properties that seems to be important in the potential treatment of disorders associated with central nervous system such as Alzheimer’s disease. The aim of this study was to effect directed detection (EDD) of biologically active substances in the root and rhizome of R. rosea L. based on chemical and biological assays performed directly on a thin-layer chromatography (TLC) plate. TLC-direct bioautography revealed the presence of both antioxidants (DPPH assay) and antibacterials (Bacillus subtilis assay) in the plant methanol extract. Unfortunately, the presence of acetylcholinesterase (AChE) inhibitors was not evident. The marker compounds, typical for R. rosea L. that is salidroside, rosavin, p-tyrosol, and hydroquinone were searched in the extract using TLC densitometry. In fact, only rosavin was found in the investigated extract. Graphical Abstract

中文翻译：

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红景天根和根茎提取物的效果定向检测

摘要 红景天是一种众所周知的适应原植物，具有抗氧化、抗抑郁、抗压力和抗痴呆的特性，在治疗与中枢神经系统相关的疾病（如阿尔茨海默病）方面似乎很重要。本研究的目的是基于直接在薄层色谱 (TLC) 板上进行的化学和生物测定，对蔷薇的根和根茎中的生物活性物质进行定向检测 (EDD)。TLC 直接生物自显影显示植物甲醇提取物中同时存在抗氧化剂（DPPH 测定）和抗菌剂（枯草芽孢杆菌测定）。不幸的是，乙酰胆碱酯酶 (AChE) 抑制剂的存在并不明显。R. rosea L. 的典型标记化合物是红景天苷、rosavin、p-酪醇、使用 TLC 光密度测定法在提取物中搜索对苯二酚和氢醌。事实上，在研究的提取物中只发现了罗沙文。图形概要