Abstract At present, the anticarcinogenic properties of nine heterocyclic thiosemicarbazone copper(II) complexes of the type [Cu(L1 − 3)2] (1–3), [Cu(L1 − 3)(bpy)]Cl (4–6), and [Cu(L1 − 3)(phen)]Cl (7–9) have been synthesized from three sulfur containing ligands such as (E)-2-(2-(benzo[d]thiazol-2-ylthio)-1-phenylethylidene)-N-methylhydrazinecarbothioamide H(L1), (E)-2-(2-(benzo[d]thiazol-2-ylthio)-1-phenylethylidene)-N-ethylhydrazinecarbothio amide H(L2) and (E)-2-(2-(benzo[d]thiazol-2-ylthio)-1-phenylethylidene)-N-phenylhydrazinecarbothioamide H(L3) with diimine co-ligands (bpy = 2,2′-bipyridyl, phen = 1,10-phenanthroline). The synthesized ligands were characterized by FT-IR, UV-vis, 1H and 13C NMR spectroscopies. All the complexes were characterized using various spectro-analytical techniques such as molar conductivity, elemental analysis and UV-vis, FT − IR and EPR spectroscopy. Also, it confirms the square planar coordination of the mixed-ligand copper(II) complexes by one deprotonated ligand and N,N′-donor of bidentate ligand while in copper(II) bis complexes coordinated by two deprotonated ligands. In addition, biological functions of the synthesized compounds have been investigated using the disc diffusion method, agarose gel electrophoresis and MTT assay. Fascinatingly, complexes with heteroaromatic moieties had potential biological activities rather than copper(II) bis complexes. This relative increase in the biological effects of copper(II) complexes is possibly due to the presence of different substituents at thioamide and phen moiety. From this study, it was concluded that the mixed-ligand copper(II) complexes could be considered as an effective chemotherapeutic drug. Graphical Abstract

中文翻译：

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带有 (E)-2-(2-(benzo[d]thiazol-2-ylthio)-1-phenylethylidene)thiosemicarbazone 和二亚胺共配体的铜 (II) 复合物的 DNA 相互作用、抗菌和体外细胞毒性特性

摘要 目前，[Cu(L1 − 3)2] (1–3), [Cu(L1 − 3)(bpy)]Cl (4–6 ) 和 [Cu(L1 − 3)(phen)]Cl (7-9) 已由三种含硫配体合成，例如 (E)-2-(2-(benzo[d]thiazol-2-ylthio) -1-苯基亚乙基)-N-甲基肼碳硫酰胺H(L1)、(E)-2-(2-(苯并[d]噻唑-2-基硫代)-1-苯基亚乙基)-N-乙基肼碳硫酰胺H(L2)和( E)-2-(2-(苯并[d]噻唑-2-基硫基)-1-苯基亚乙基)-N-苯肼碳硫酰胺H(L3)与二亚胺共配体（bpy = 2,2'-联吡啶，phen = 1 ,10-菲咯啉）。合成的配体通过 FT-IR、UV-vis、1H 和 13C NMR 光谱表征。使用各种光谱分析技术对所有配合物进行表征，例如摩尔电导率、元素分析和 UV-vis、FT - IR 和 EPR 光谱。此外，它还证实了混合配体铜（II）配合物由一个去质子化配体和双齿配体的 N，N'-供体的方形平面配位，而在铜（II）双配合物中由两个去质子化配体配位。此外，还使用圆盘扩散法、琼脂糖凝胶电泳和 MTT 法研究了合成化合物的生物学功能。令人着迷的是，具有杂芳族部分的复合物具有潜在的生物活性，而不是铜（II）双复合物。铜 (II) 配合物生物效应的这种相对增加可能是由于硫代酰胺和苯酚部分存在不同的取代基。从这项研究中，结论是混合配体铜(II)配合物可被认为是一种有效的化疗药物。图形概要