Abstract Commercially available fibrin glue is expensive and contains fibrin and thrombin from different sources. This limits the use of this glue for bonding regular wounds, increases the cost for cardiovascular surgeries and aggravates the risk of inflammatory and immune systemic allergic reactions. This study aims to develop a stronger and affordable fibrin glue synthesized from the patient’s own plasma within a few minutes. Fibrin glue was synthesized by combining calcium chloride with human plasma and a gel-like adhesive was prepared for direct application. Different properties and morphological features of the autologous glue along with the fiber-supported one were evaluated for different concentrations (5, 10 and 20% w/v) of calcium chloride. 10% calcium chloride was found as the optimum with clotting time varying from 8 to 25 min for frozen plasma and 3 min for freshly prepared plasma. The average adhesion strength was 46.2, 55.8 and 40.5 g/cm2 for 5, 10 and 20% calcium chloride, respectively. The adhesion strength increased up to 74.8 g/cm2 due to the addition of 1 mL of 0.5% cellulose that addresses the issue of weaker adhesion strength found in typical autologous glue. The average water content was the lowest for 10% calcium chloride and was reduced to 15.3% after cellulose addition. The proposed fibrin glue has high adhesion strength like the commercial ones and can be prepared in a few minutes. This affordable, enhanced fibrin glue has the potential to be used in a wide range of applications without the risk of possible inflammatory and allergic reactions.

中文翻译：

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从人血浆中开发天然纤维支持的高强度自体纤维蛋白胶

摘要 市售的纤维蛋白胶价格昂贵，并且含有来自不同来源的纤维蛋白和凝血酶。这限制了这种胶水用于粘合常规伤口的使用，增加了心血管手术的成本，并加剧了炎症和免疫系统过敏反应的风险。这项研究旨在开发一种在几分钟内从患者自己的血浆中合成的更强且价格合理的纤维蛋白胶。通过将氯化钙与人血浆结合合成纤维蛋白胶，并制备了可直接应用的凝胶状粘合剂。对于不同浓度（5、10 和 20% w/v）的氯化钙，评估了自体胶和纤维支撑胶的不同性质和形态特征。发现 10% 氯化钙是最佳的，冷冻血浆的凝血时间为 8 到 25 分钟，新鲜制备的血浆为 3 分钟。对于 5%、10% 和 20% 氯化钙，平均粘合强度分别为 46.2、55.8 和 40.5 g/cm2。由于添加了 1 mL 0.5% 纤维素，解决了典型自体胶中粘合强度较弱的问题，粘合强度增加至 74.8 g/cm2。10%氯化钙的平均含水量最低，加入纤维素后降至15.3%。所提出的纤维蛋白胶与商业胶一样具有高粘合强度，并且可以在几分钟内制备。这种经济实惠的增强型纤维蛋白胶有可能用于广泛的应用，而不会产生炎症和过敏反应的风险。