Ethidium Bromide (EtBr) is an organic compound used in molecular biology investigations. EtBr ability of intercalating in the DNA molecule makes it a toxic substance. The objective was to evaluate the phycoremediation potentials of Chlorella vulgaris, Desmodesmus subspicatus and Raphidocelis subcapitata tested separately and in a mixture (Mix) for EtBr removal from the aqueous medium. Experiments were conducted using an initial algae biomass of 10⁶ cell/mL, exposed to 500 µg/L of EtBr. The removal efficiency (µg EtBr L⁻¹) after 3 h in each treatment were: Mix (72.8 µg.L⁻¹) >D. subspicatus (48.4 µg.L⁻¹) >R. subcapitata (24.6 µg.L⁻¹) >C. vulgaris (19.9 µg.L⁻¹). However, when EtBr mass reduction per microalgae density is considered (ng.algae⁻¹), the efficiency ranking changes to: D. subspicatus (1.9 × 10⁻⁵ng.algae⁻¹) >C. vulgaris (1.4 × 10⁻⁵ng.algae⁻¹) >Mix (9.8 × 10⁻⁶ng.algae⁻¹) >R. subcapitata (2.8 × 10⁻⁶ng.algae⁻¹). The results suggest that initial algal population density is a determinant factor for efficient EtBr removal by microalgae species in short term treatments. In order to obtain 100% of EtBr removal, it should be necessary 10¹⁰, 10¹⁰ and 10¹¹ algae.mL⁻¹ of C. vulgaris, D. subspicatus and R. subcapitata, respectively. The results strongly suggest phycoremediation can be explored as an alternative method for EtBr removal.

溴化乙锭（EtBr）是一种用于分子生物学研究的有机化合物。EtBr插入DNA分子的能力使其成为有毒物质。的目的是评估的phycoremediation电位小球藻，链带藻subspicatus而言和Raphidocelis subcapitata单独地并且用于从含水介质中除去的EtBr的混合物（混合）进行测试。使用暴露于500 µg / L EtBr的初始藻类生物量10 ⁶细胞/ mL进行实验。每次处理3小时后的去除效率（µg EtBr L ^-1）为：混合物（72.8 µg.L ^-1）>副角毛盘菌（48.4 µg.L ^-1）>亚头衣藻（24.6微克L ^-1）>寻常梭状芽胞杆菌（19.9微克L ^-1）。但是，当考虑到每微藻密度的EtBr质量降低（ng.algae ^-1）时，效率等级将变为：D. subspicatus（1.9×10 ^-5 ng.algae ^-1）> C. v 。（1.4×10 ^-5） ng.algae ^-1）>混合（9.8×10 ^-6 ng.algae ^-1）> subcapitata（2.8×10 ^-6 ng.algae ^-1）。结果表明，初始藻类种群密度是短期治疗中微藻类有效去除EtBr的决定性因素。为了获得的EtBr去除100％，应该10是必要的¹⁰，10 ¹⁰和10 ¹¹ algae.mL ^-1的小球藻，D. subspicatus而言和，R. subcapitata分别。结果强烈表明，phycoremediation可以探索为替代EtBr去除方法。