Introduction: After the genomic era, the analysis of the proteome has gained increasing importance. Peptides and/or proteins present in tissue or body fluids can depict health and are prone to change during disease, not only in configuration but also in abundance. Early on, high throughput proteome analysis was implemented in the diagnostic of therapy-linked or induced complications arising after allogeneic hematopoietic stem cell transplantation (HSCT). Several proteomic approaches are currently used in the prediction or diagnosis of acute and/or chronic graft-versus-host disease (GvHD).Areas covered: This review will report on two high throughput proteomics technologies used in the clinical setting to date, namely enzyme-linked-immunosorbent assays (ELISA) for key proteins involved in the pathogenesis of acute GvHD and on capillary electrophoresis coupled on-line to mass spectrometry (CE-MS). Here, we summarize the current data and discuss the strength as well as the limitations of each method and compare the usefulness and practicability in the post-HSCT setting for prediction and diagnosis of acute GvHD.Expert commentary: Both technologies are applied in the clinic and have been tested on several hundred patients after HSCT. The data from both technologies may complement each other in diagnosis of GvHD.

中文翻译：

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造血干细胞移植的蛋白质组学。

简介：在基因组时代之后，蛋白质组分析变得越来越重要。组织或体液中存在的肽和/或蛋白质可以描述健康状况，并且在疾病期间容易发生变化，不仅在配置上而且在数量上。早期，高通量蛋白质组分析用于诊断同种异体造血干细胞移植 (HSCT) 后出现的治疗相关或诱发并发症。目前有几种蛋白质组学方法用于预测或诊断急性和/或慢性移植物抗宿主病 (GvHD)。即酶联免疫吸附测定 (ELISA)，用于检测参与急性 GvHD 发病机制的关键蛋白质，以及在线质谱联用 (CE-MS) 的毛细管电泳。在这里，我们总结了目前的数据并讨论了每种方法的优势和局限性，并比较了造血干细胞移植后环境中预测和诊断急性 GvHD 的有用性和实用性。 专家评论：这两种技术都应用于临床和已在 HSCT 后对数百名患者进行了测试。两种技术的数据可以在 GvHD 的诊断中相互补充。我们总结了目前的数据并讨论了每种方法的优势和局限性，并比较了造血干细胞移植后环境中预测和诊断急性 GvHD 的有用性和实用性。 专家评论：这两种技术都已应用于临床并已得到在 HSCT 后对数百名患者进行了测试。两种技术的数据可以在 GvHD 的诊断中相互补充。我们总结了目前的数据并讨论了每种方法的优势和局限性，并比较了造血干细胞移植后环境中预测和诊断急性 GvHD 的有用性和实用性。 专家评论：这两种技术都已应用于临床并已得到在 HSCT 后对数百名患者进行了测试。两种技术的数据可以在 GvHD 的诊断中相互补充。