ABSTRACT Vanadium-dependent haloperoxidases (V-HPO) are specific enzymes involved in the metabolism of reactive oxygen species in marine algae. These enzymes contribute to early embryogenesis of algae in the order Fucales, catalyzing the reactions which result in phlorotannin incorporation into the newly forming zygote cell wall and adhesive material. In this study we showed that early embryogenesis in Fucus vesiculosus is accompanied by synchronous changes of V-HPO activity, hydrogen peroxide content and content of cell-wall-associated phlorotannins. Phlorotannin incorporation into the cell wall of Fucus zygotes had already begun 1 h after fertilization, and this process was accompanied by rapid increase of H2O2 content and V-HPO activity. We suggest that altogether this initiates polymerization of phenolics and their cross-linking with the major cell wall constituent, alginic acid. Presumably, de novo phlorotannin biosynthesis is involved in this process, as GC-MS analysis showed dramatic changes in content of intracellular phloroglucinol and low-molecular-weight phlorotannins during the first 9 days of F. vesiculosus embryogenesis.

中文翻译：

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在 Fucus vesiculosus (Phaeophyceae) 早期胚胎发生过程中，钒依赖性卤代过氧化物酶活性和根皮单宁掺入细胞壁

摘要 钒依赖性卤代过氧化物酶 (V-HPO) 是参与海藻中活性氧代谢的特定酶。这些酶有助于藻类在 Fucales 目中的早期胚胎发生，催化导致根皮单宁掺入新形成的受精卵细胞壁和粘附材料的反应。在这项研究中，我们发现 Fucus vesiculosus 的早期胚胎发生伴随着 V-HPO 活性、过氧化氢含量和细胞壁相关根皮单宁含量的同步变化。受精后 1 小时，根皮单宁已开始掺入墨角藻受精卵的细胞壁，这一过程伴随着 H2O2 含量和 V-HPO 活性的快速增加。我们认为这会引发酚类物质的聚合以及它们与主要细胞壁成分海藻酸的交联。据推测，该过程涉及从头根皮单宁生物合成，因为 GC-MS 分析显示，在 F. vesiculosus 胚胎发生的前 9 天，细胞内间苯三酚和低分子量根皮单宁的含量发生了巨大变化。