SummaryTransparentCasperzebrafish allow studies of vertebrate sexual maturation and gonad developmentin vivo.Caspergonad dynamics can be observed longitudinally over time and non-invasively. Gonad maturation and reproduction are complex processes subject to disruption by endocrine-disrupting chemicals (EDCs), such as diethylstilbestrol (DES). DES was used as a ‘proof of principle’ to ascertain the usefulness of theCaspermodel to determine EDC effects on gonad maturation. Puberty onset in control juvenileCasperzebrafish (N= 43) averaged 13.2 weeks post fertilization (WPF) for females and included increased vent size, while in males puberty occurred at 11.7 WPF along with maintenance of small vents. DES treatment for 6 days in early juveniles (N= 20) induced an average delay in puberty of 5 weeks in females and 10 weeks in males. DES induced loss of breeding tubercles and vent enlargement in post-pubescent males. Puberty in control fish was correlated with an average body length of 1.7 cm for males and 1.8 cm for females. Increased testes opacity, small vent and breeding tubercles denoted male puberty. Puberty in females was defined as ovarian follicle diameters reaching 400 µm with increasingly opaque follicles and by an increased vent size. These results are like those for wild-type zebrafish and indicate that theCaspermodel is a useful system for studying gonad dynamicsin vivo. Future use of transgenic reporter lines inCasperwill allow new avenues of investigation into the reproductive biology of this vertebrate model.

中文翻译：

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青春期可视化：透明卡斯珀斑马鱼的性成熟

摘要透明卡斯帕斑马鱼允许研究脊椎动物性成熟和性腺发育体内.卡斯帕性腺动力学可以随着时间的推移和非侵入性的纵向观察。性腺成熟和繁殖是一个复杂的过程，会受到干扰内分泌的化学物质 (EDC) 的干扰，例如己烯雌酚 (DES)。DES 被用作“原理证明”来确定卡斯帕模型来确定 EDC 对性腺成熟的影响。控制青少年的青春期开始卡斯帕斑马鱼 (ñ= 43) 女性受精后 (WPF) 平均为 13.2 周，包括增加的排气口尺寸，而男性的青春期发生在 11.7 WPF，同时维持小排气口。早期青少年 DES 治疗 6 天 (ñ= 20) 导致女性青春期平均延迟 5 周，男性平均延迟 10 周。DES 在青春期后的雄性中诱导繁殖结节的丧失和排气口扩大。对照鱼的青春期与雄性平均体长 1.7 厘米和雌性 1.8 厘米相关。睾丸混浊增加，小孔和繁殖结节表示男性青春期。女性青春期被定义为卵巢卵泡直径达到 400 µm，卵泡越来越不透明，并且排气口尺寸增加。这些结果与野生型斑马鱼的结果相似，表明卡斯帕模型是研究性腺动力学的有用系统体内. 转基因报告基因系的未来用途卡斯帕将为研究这种脊椎动物模型的生殖生物学开辟新途径。