Plant cyclophilin (CYP) is related to chloroplast photoprotection, redox and other growth and developmental regulatory processes and responds to stress and improves tolerance to saline–alkali stress. Besides, it exerts peptidyl-prolyl cis/trans isomerase activity to participate in protein synthesis and folding. In this study, Northern blot was used to analyse the CsCYP1A gene (a CYP gene isolated from a Chlorella species) (accession number: KY207381) of tolerant Chlorella vulgaris in soda saline–alkali soil. The result showed that the expression of the CsCYP1A gene was induced by NaHCO3, NaCl and sorbitol. Additionally, Agrobacterium tumefaciens was used to infect the callus of rice (Oryza sativa var. Longjing11) for genetic transformation. Molecular detection confirmed that transgenic seedlings overexpressing CsCYP1A were obtained by hygromycin selection. Treatment with NaCl and NaHCO3 at the five-leaf stage was performed in the seedlings, and the results showed that there were significant differences between the CsCYP1A overexpressed rice lines and non-transgenic (NT) Longjing11 in terms of plant height, fresh weight, chlorophyll content, malondialdehyde content and ascorbate peroxidase activity. The CsCYP1A overexpression rice lines had higher tolerance to NaCl and NaHCO3 than NT. The current findings indicate that CsCYP1A can enhance the tolerance of rice to saline–alkali stress possibly through its involvement in reactive oxygen scavenging pathways.

中文翻译：

---

碱性小球藻CsCYP1A基因过表达水稻耐盐碱分析

植物亲环素 (CYP) 与叶绿体光保护、氧化还原和其他生长发育调节过程有关，对胁迫作出反应并提高对盐碱胁迫的耐受性。此外，它还发挥肽基脯氨酰顺式/反式异构酶活性参与蛋白质合成和折叠。在本研究中，Northern blot 用于分析CsCYP1A基因（一个CYP基因分离自小球藻种）（登录号：KY207381）的耐受小球藻在苏打盐碱土中。结果表明，表达CsCYP1ANaHCO 诱导基因3, NaCl 和山梨糖醇。此外，根癌农杆菌用于感染水稻愈伤组织（水稻变种。Longjing11) 用于遗传转化。分子检测证实转基因幼苗过表达CsCYP1A通过潮霉素筛选获得。用 NaCl 和 NaHCO 处理3在幼苗中进行了五叶期，结果表明两者之间存在显着差异CsCYP1A在株高、鲜重、叶绿素含量、丙二醛含量和抗坏血酸过氧化物酶活性方面过表达的水稻品系和非转基因（NT）龙井11。这CsCYP1A过表达水稻对 NaCl 和 NaHCO 的耐受性更高3比新台币。目前的研究结果表明CsCYP1A可以通过参与活性氧清除途径增强水稻对盐碱胁迫的耐受性。