In this study, sows were fed 200 (LD), 800 (ND) and 3200 (HD) IU of vitamin D3/kg basal diet during pregnancy (from 41 d to birth), respectively. All their offspring pigs were fed the same vitamin D3 replete die. At 150 days of age, a total of 18 offspring pigs (six offspring pigs per maternal diet group, sex balance) were weighed and slaughtered to investigate effects of maternal vitamin D3 during pregnancy on fatty acids synthase (FASN) and hormone-sensitive lipase (LIPE) expression in offspring pigs. The results showed that LD offspring pigs had higher FASN mRNA expression and the ratio of FASN/LIPE mRNA expression in subcutaneous adipose tissue, as well as higher LIPE mRNA expression of longissimus dorsal muscle, whereas, had lower the ratio of FASN/LIPE mRNA expression in longissimus dorsal muscle compared with ND or HD offspring pigs, respectively. Meanwhile, LD offspring pigs had higher carcass fat, average backfat thickness (ABFT), serum insulin and leptin levels, lower intramuscular fat (IMF), serum free fatty acid and triglycerol levels compared with ND or HD offspring pigs. In addition, the ratio of FASN/LIPE mRNA expression was negatively correlated with IMF content, and positively correlated to carcass fat content and ABFT in offspring pigs. Meanwhile, FASN mRNA expression was positively correlated with carcass fat content, while negatively correlated with ABFT in offspring pigs. These results suggested that maternal vitamin D3 affected fat accumulation and meat quality by regulating FASN and LIPE mRNA expression in offspring pigs.

中文翻译：

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孕期母体维生素D3对仔猪FASN和LIPE mRNA表达的影响

在这项研究中，母猪饲喂了 200 (LD)、800 (ND) 和 3200 (HD) IU 的维生素 D3/kg 怀孕期间的基础饮食（从 41 天到出生），分别。他们所有的后代猪都喂食了相同的维生素 D3充满死亡。在 150 日龄时，共称重和屠宰 18 头后代猪（每个母体饮食组 6 头后代猪，性别平衡），以研究母体维生素 D 的影响3怀孕期间对脂肪酸合成酶（FASN) 和激素敏感性脂肪酶 (利普) 在后代猪中的表达。结果表明，LD后代猪具有较高的FASNmRNA表达与比例FASN/LIPEmRNA在皮下脂肪组织中的表达，以及更高的利普mRNA表达最长肌而背肌的比例较低FASN/LIPEmRNA表达在最长肌背肌分别与 ND 或 HD 后代猪相比。同时，与ND或HD后代猪相比，LD后代猪的胴体脂肪、平均背膘厚度（ABFT）、血清胰岛素和瘦素水平、肌内脂肪（IMF）、血清游离脂肪酸和甘油三酯水平较低。此外，比例FASN/LIPEmRNA表达与子代猪的IMF含量呈负相关，与胴体脂肪含量和ABFT呈正相关。同时，FASNmRNA表达与胴体脂肪含量呈正相关，而与后代猪的ABFT呈负相关。这些结果表明，母体维生素 D3通过调节影响脂肪积累和肉质FASN和利普后代猪中的 mRNA 表达。