Thromboembolic disorders are the second leading cause of death in breast cancer. Antiplatelet therapy combined with cancer therapy is a potential treatment strategy against cancer-associated thromboembolic disorders; however, the efficacy of such dual treatment has not been established. This study reports novel findings on the response of hormone-dependent breast cancer cell lines (MCF7/T47D) following 24 h treatment with Anastrozole, combined with Aspirin and Clopidogrel cocktail; and Atopaxar. Neutral red and lactate dehydrogenase assays were conducted to assess viability and cytotoxicity respectively. Flow cytometric Annexin-V/PI assay was used to assess the mode of cell death. Morphological alterations were studied using scanning electron microscopy. Statistical analysis was conducted using Statistica V13. Definitive outcomes were established with flow cytometric detection of phosphatidylserine exposure and propidium iodide staining, complemented with ultrastructural analysis. Results showed that a few cells were undergoing death mainly through secondary necrosis. Morphological features suggesting induced cell motility (pseudopodia/ruffled membranes) were observed in both cell lines; notably, T47D cells presented pronounced features than MCF7 cells. Overall, these findings suggest that such combined treatment may differentially promote cell survival, inducing a more aggressive breast cancer phenotype.

中文翻译：

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阿那曲唑和抗血小板疗法联合治疗可促进乳腺癌细胞存活

血栓栓塞性疾病是导致乳腺癌死亡的第二大原因。抗血小板治疗与癌症治疗相结合是针对癌症相关血栓栓塞性疾病的潜在治疗策略；然而，这种双重治疗的功效尚未确定。本研究报告了激素依赖性乳腺癌细胞系 (MCF7/T47D) 在阿那曲唑联合阿司匹林和氯吡格雷鸡尾酒治疗 24 小时后的反应的新发现；和阿托帕沙。进行中性红和乳酸脱氢酶测定以分别评估活力和细胞毒性。流式细胞仪膜联蛋白-V/PI 测定用于评估细胞死亡的方式。使用扫描电子显微镜研究形态学改变。使用 Statistica V13 进行统计分析。通过流式细胞术检测磷脂酰丝氨酸暴露和碘化丙啶染色，并辅以超微结构分析，确定了最终结果。结果表明，少数细胞主要通过继发性坏死而死亡。在两种细胞系中均观察到表明诱导细胞运动（假足/褶皱膜）的形态学特征；值得注意的是，T47D 细胞比 MCF7 细胞具有明显的特征。总体而言，这些研究结果表明，这种联合治疗可能会不同程度地促进细胞存活，从而诱导更具侵袭性的乳腺癌表型。在两种细胞系中均观察到表明诱导细胞运动（假足/褶皱膜）的形态学特征；值得注意的是，T47D 细胞比 MCF7 细胞具有明显的特征。总体而言，这些研究结果表明，这种联合治疗可能会不同程度地促进细胞存活，从而诱导更具侵袭性的乳腺癌表型。在两种细胞系中均观察到表明诱导细胞运动（假足/褶皱膜）的形态学特征；值得注意的是，T47D 细胞比 MCF7 细胞具有明显的特征。总体而言，这些研究结果表明，这种联合治疗可能会不同程度地促进细胞存活，从而诱导更具侵袭性的乳腺癌表型。