Abstract

To shorten the duration of upper respiratory tract (URT) diseases or reduce the symptoms alternative treatments to antibiotics and decongestants are needed, due to their side effects and frequent failure. Recent human microbiome research has led to the hypothesis that beneficial bacteria, capable of colonizing the human URT, could constitute an interesting approach to prevent and reduce URT diseases. Since probiotics are live microorganisms with a beneficial effect on the host, it is important that the probiotic bacteria are delivered at the site of action, without hampering their viability. Here, the formulation applicability of eight new probiotic candidates belonging to the Lactobacillus genus was evaluated after spray drying. Besides viability, growth and fermentation abilities after drying were confirmed through experiments. A promising strain was chosen for further optimization. Microscopic visualization and flow cytometry of bacterial morphology identified spray drying as a suitable processing method. Adhesion experiments indicated no changes in functionality. Lastly, the contact time between bacteria and hot drying air was identified as a crucial parameter in spray drying of probiotics.

摘要

为了缩短上呼吸道 (URT) 疾病的持续时间或减轻症状，需要抗生素和减充血剂的替代疗法，因为它们的副作用和频繁失败。最近的人类微生物组研究提出了一种假设，即能够定植人类 URT 的有益细菌可以构成一种有趣的方法来预防和减少 URT 疾病。由于益生菌是对宿主有有益作用的活微生物，重要的是将益生菌输送到作用部位，而不妨碍它们的生存能力。在这里，属于乳杆菌属的八种新益生菌候选物的配方适用性喷雾干燥后评价属。除了生存能力外，干燥后的生长和发酵能力也通过实验得到证实。选择了一种有前途的菌株进行进一步优化。细菌形态的显微可视化和流式细胞术确定喷雾干燥是一种合适的处理方法。粘附实验表明功能没有变化。最后，细菌与热干燥空气之间的接触时间被确定为益生菌喷雾干燥的关键参数。