Abstract

Lobularia maritima (Alyssum maritimum, Brassicaceae), commonly known as sweet alyssum, is an annual ornamental halophyte widely spread along the Tunisian seashore. Lobularia maritima leaf ethanol extract was tested in an experimental model of hepatotoxicity induced by carbon tetrachloride (CCl₄). L. maritima extract was found to possess in vitro antioxidant activity by scavenging the DPPH radical (IC₅₀= 45 μg/mL), reducing/chelating iron ions and inhibiting liver lipid peroxidation induced by FeSO₄. The levels of total phenolics and flavonoids were 175 ± 2.66 mg GAE/g, and 35 ± 2.88 mg QE/g respectively. Moreover, HPLC analysis revealed six compounds, namely gallic, salicylic, ellagic and ferulic acids as well as catechin and quercetin. A mice model of acute liver injury was successfully established after a single intraperitoneal injection of CCl₄, as evidenced by histological analysis, Masson trichrome and Sirius red staining. Compared with the CCl₄ intoxicated group, the L. maritima treatment resulted to reduce the liver serum marker enzymes, lipid peroxidation and increased the activities of antioxidant enzymes with further amelioration in the oxidative stress. The present findings discover the therapeutic potentials of L. maritima empowered with promising natural leads for the treatment of oxidative stress associated health disorders by attenuating free radicals, inhibiting lipid peroxidation, and upregulating the tissue-specific antioxidant enzymes.

摘要

Lobularia maritima ( Alyssum maritimum , Brassicaceae)，俗称甜香雪草，是一种一年生观赏盐生植物，广泛分布于突尼斯海滨。在四氯化碳 (CCl _{4 ) 诱导的肝毒性实验模型中测试了}海小叶的乙醇提取物。L. maritima提取物通过清除 DPPH 自由基 (IC _{50 = 45 μg/mL)、还原/螯合铁离子和抑制 FeSO 4}诱导的肝脏脂质过氧化而具有体外抗氧化活性. 总酚和类黄酮的水平分别为 175 ± 2.66 mg GAE/g 和 35 ± 2.88 mg QE/g。此外，HPLC分析揭示了六种化合物，即没食子酸、水杨酸、鞣花酸和阿魏酸以及儿茶素和槲皮素。经组织学分析、马松三色和天狼星红染色证实，单次腹腔注射CCl ₄后成功建立小鼠急性肝损伤模型。与CCl ₄中毒组相比，L. maritima处理导致肝脏血清标志酶、脂质过氧化降低，抗氧化酶活性增加，进一步改善了氧化应激。目前的研究结果发现了L. maritima的治疗潜力通过减弱自由基、抑制脂质过氧化和上调组织特异性抗氧化酶，赋予治疗氧化应激相关健康疾病的有希望的天然先导物。