Abstract

In this study, the hepatoprotective activity of methanol bark extract of Alangium salviifolium (BEA) was evaluated for biochemical and histological parameters in swiss albino mice with CCl₄-induced hepatotoxicity. The hepatomodulatory effect of two doses of BEA (20 and 50 mg/kg bw for 15 days by oral gavage) was assessed on antioxidant enzymes, phase I and phase II drug detoxifying enzymes. For the characterization of the extract, GC-MS analysis was performed that revealed the abundance of alkaloids and steroidal compounds. Total phenolic and flavonoid contents in BEA were 69.61 ± 0.18 mg GAE/g and 46.27 ± 3.44 mg Rutin/g, respectively. BEA administration decreased the levels of AST, ALT, and ALP, which were elevated due to hepatic damage by CCl₄. BEA significantly decreased the lipid peroxidation, activities of LDH, and phase I enzymes including cytochrome P450 reductase, cytochrome b5 reductase while increased the activities of SOD, CAT, and phase II enzymes DT-diaphorase and glutathione S-transferase in liver. Further, histological evaluation of the liver tissue was suggestive of the protective effect of BEA against CCl₄ toxicity. Together, these results suggest that BEA has strong hepatoprotective activity in mice which may also be attributed to its potential chemopreventive efficacy.

摘要

在这项研究中，对具有 CCl ₄诱导肝毒性的瑞士白化病小鼠的生化和组织学参数评估了丹参甲醇树皮提取物 (BEA)的保肝活性。在抗氧化酶、I 期和 II 期药物解毒酶方面评估了两剂 BEA（20 和 50 毫克/公斤体重，口服管饲法，持续 15 天）的肝脏调节作用。为了表征提取物，进行了 GC-MS 分析，揭示了生物碱和甾体化合物的丰度。BEA 中的总酚类和类黄酮含量分别为 69.61 ± 0.18 mg GAE/g 和 46.27 ± 3.44 mg Rutin/g。BEA 给药降低了 AST、ALT 和 ALP 的水平，这些水平由于 CCl _{4的肝损伤而升高}. BEA显着降低了肝脏中脂质过氧化、LDH和I期酶（包括细胞色素P450还原酶、细胞色素b5还原酶）的活性，同时增加了SOD、CAT和II期酶DT-心肌黄酶和谷胱甘肽S-转移酶的活性。此外，肝组织的组织学评价提示BEA对CCl ₄毒性的保护作用。总之，这些结果表明 BEA 在小鼠中具有很强的保肝活性，这也可能归因于其潜在的化学预防功效。