Abstract

Natural extracts of strawberry guava leaf were obtained using the following techniques: supercritical CO₂ extraction (at 14, 17, and 20 MPa and 35, 45, and 55 °C); dynamic maceration with hexane solvent; Soxhlet extraction; ultrasound-assisted extraction (at 40, 50, and 60 °C; 100, 300, and 500 W; 1:10, 15, and 20 g mL⁻¹), and supercritical extraction followed by ultrasound. Yield results were 3.59% for Soxhlet extraction, 2.68% for ultrasound-assisted extraction, 1.46% for ultrasound re-extraction after supercritical extraction, 0.98% for dynamic maceration, and 0.86% for supercritical CO₂ extraction. Natural extracts of strawberry guava leaves obtained via supercritical extraction were found to be rich in eudesmol, squalene, and vitamin E, in 11.03, 19.44, and 53.42% percentages, respectively. They also exhibited high antioxidant activity, 26.15 μg mL⁻¹. This result was directly related to the α-tocopherol present in vitamin E.

摘要

草莓番石榴叶的天然提取物使用以下技术获得：超临界 CO ₂提取（在 14、17 和 20 MPa 和 35、45 和 55 °C 下）；用己烷溶剂动态浸渍；索氏提取；超声辅助萃取（在 40、50 和 60 °C；100、300 和 500 W；1:10、15 和 20 g mL ^-1）和超临界萃取，然后进行超声。索氏提取的产率为 3.59%，超声辅助提取的产率为 2.68%，超临界萃取后超声再提取的产率为 1.46%，动态浸渍的产率为 0.98%，超临界 CO _{2 的产}率为 0.86%萃取。发现通过超临界萃取获得的草莓番石榴叶天然提取物分别富含 eudesmol、角鲨烯和维生素 E，百分比分别为 11.03、19.44 和 53.42%。它们还表现出高抗氧化活性，26.15 μg mL ^-1。这一结果与维生素 E 中存在的 α-生育酚直接相关。