ABSTRACT

We investigated the antiproliferative and apoptosis inducing effects of a methanolic extract of Centaurea hermannii in Vero and HeLa cell lines. Both cell lines also were treated with doxorubicin. Antiproliferative effects were assessed by MTT assay and cell index parameters were determined using the xCELLigence real time cell analysis system (RTCA). The IC₅₀, the half maximal inhibitory concentration, and selectivity index (SI) values for doxorubicin and the C. hermannii extract were determined for both cell lines. At 48 h culture, the C. hermannii extract exhibited a potent cytotoxic effect in HeLa cells. Our MTT findings were consistent with morphological evaluation and analysis by the xCELLigence RTCA. The apoptosis inducing effect of the C. hermannii extract in HeLa cells was determined by flow cytometry; caspases 3, 7 and 9 activation assays; and quantitative reverse transcription polymerase chain reaction analysis. Our findings suggest that components of C. hermannii extract should be investigated as possible anticancer drugs.

摘要

我们研究了矢车菊的甲醇提取物在Vero和HeLa细胞系中的抗增殖和凋亡诱导作用。两种细胞系也用阿霉素处理。通过MTT测定评估抗增殖作用，并使用xCELLigence实时细胞分析系统（RTCA）确定细胞指数参数。确定了两种细胞系的阿霉素和赫氏梭菌提取物的IC ₅₀，半数最大抑制浓度和选择性指数（SI）值。在培养48小时后，C。hermannii提取物在HeLa细胞中表现出有效的细胞毒性作用。我们的MTT结果与xCELLigence RTCA的形态学评估和分析结果一致。流式细胞术测定了曼氏梭状芽胞杆菌提取物在HeLa细胞中的凋亡诱导作用。半胱氨酸蛋白酶3、7和9激活测定；和定量逆转录聚合酶链反应分析。我们的研究结果表明，应将C.hermannii提取物的成分作为可能的抗癌药物进行研究。