ABSTRACT

Neural crest cells possess characteristics of stem cells including plasticity and ability to differentiate into various cell types. HNK1 and Sox10 are markers of neural crest cell progenitors that have been demonstrated in osteoblasts during osteogenesis of the maxilla and mandible. We investigated the presence of Sox10 and HNK1 during regeneration of mandibular bone defects. Defects were created in mandibles of rats. Samples of these defects were collected at 7, 14 and 28 days post-surgery; bone regeneration was observed during this period. Immunohistochemical analysis revealed expression of HNK1 and Sox10 in osteoblasts, osteocytes and osteogenic cells, whereas osteoclasts were unstained. HNK1 expression was increased in osteoblasts and osteocytes over time and SOX10 expression was found in osteoblasts and osteogenic cells at 7, 14 and 28 days post-surgery. HNK1 and Sox10 are present in osteoblasts, osteocytes and osteogenic cells during mandible bone regeneration.

摘要

神经rest细胞具有干细胞的特征，包括可塑性和分化为各种细胞类型的能力。HNK1和Sox10是神经c细胞祖细胞的标志物，已在上颌骨和下颌骨的成骨过程中在成骨细胞中得到证实。我们调查了下颌骨缺损的再生过程中Sox10和HNK1的存在。在大鼠的下颌骨中产生了缺陷。在手术后第7、14和28天收集这些缺陷的样品。在此期间观察到骨再生。免疫组织化学分析显示HNK1和Sox10在成骨细胞，成骨细胞和成骨细胞中表达，而破骨细胞未染色。随着时间的推移，成骨细胞和成骨细胞中的HNK1表达增加，而成骨细胞和成骨细胞中的SOX10表达则随时间的推移而增加（7岁）手术后14和28天。HNK1和Sox10存在于下颌骨再生过程中的成骨细胞，成骨细胞和成骨细胞中。