Abstract S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) play an important role in various biochemical reactions involving the maintenance of human health and disease prevention. In this study, an accurate, rapid, economical, and simple protocol is reported for the simultaneous determination of the SAM and SAH concentrations in human urine. 97% trifluoroacetic acid (0.05%) was used in the mobile phase as a micromolecule ion-pairing agent in order to enhance the formation of ion pairs and the protonation of the SAM and SAH analytes. Following solid phase extraction (SPE), the separation of SAM and SAH was performed by micromolecule ion pair chromatography using a C18 column. Three types of solid phase extraction columns were characterized for the purification of the urine samples, and their correlative mechanisms were evaluated in detail. Good linearity values were obtained across the concentration ranges from 0.5 to 60 μmol/L and 0.1 to 10 μmol/L for SAM and SAH, respectively. The limits of detection were equal to 0.006 and 0.01 μmol/L for SAM and SAH, respectively, while the limits of quantitation were 0.02 and 0.04 μmol/L for these analytes. The intra-day and inter-day precision values for the determined SAM and SAH species were less than 1.38. The recovery values of SAM and SAH in urine were between 83.65 and 98.41%. Thus, this method has demonstrated to be suitable for the determination of SAM and SAH in human urine.

中文翻译：

---

基于小分子离子对试剂的固相萃取离子色谱法测定人尿液中的S-腺苷甲硫氨酸和S-腺苷高半胱氨酸

摘要 S-腺苷甲硫氨酸（SAM）和S-腺苷高半胱氨酸（SAH）在涉及维持人类健康和疾病预防的各种生化反应中发挥着重要作用。在这项研究中，报告了一种准确、快速、经济且简单的方案，用于同时测定人类尿液中的 SAM 和 SAH 浓度。在流动相中使用 97% 三氟乙酸 (0.05%) 作为小分子离子对试剂，以增强离子对的形成以及 SAM 和 SAH 分析物的质子化。在固相萃取 (SPE) 之后，使用 C18 柱通过小分子离子对色谱分离 SAM 和 SAH。三种类型的固相萃取柱用于尿液样品的纯化，并对它们的相关机制进行了详细评估。SAM 和 SAH 分别在 0.5 至 60 μmol/L 和 0.1 至 10 μmol/L 的浓度范围内获得了良好的线性值。SAM 和 SAH 的检测限分别为 0.006 和 0.01 μmol/L，而这些分析物的定量限分别为 0.02 和 0.04 μmol/L。确定的 SAM 和 SAH 种类的日内和日间精度值小于 1.38。尿液中SAM和SAH的回收率在83.65-98.41%之间。因此，该方法已证明适用于测定人尿中的 SAM 和 SAH。SAM 和 SAH 的浓度分别为 01 μmol/L，而这些分析物的定量限分别为 0.02 和 0.04 μmol/L。确定的 SAM 和 SAH 种类的日内和日间精度值小于 1.38。尿液中SAM和SAH的回收率在83.65-98.41%之间。因此，该方法已证明适用于测定人尿中的 SAM 和 SAH。SAM 和 SAH 的浓度分别为 01 μmol/L，而这些分析物的定量限分别为 0.02 和 0.04 μmol/L。确定的 SAM 和 SAH 种类的日内和日间精度值小于 1.38。尿液中SAM和SAH的回收率在83.65-98.41%之间。因此，该方法已证明适用于测定人尿中的 SAM 和 SAH。