Abstract In the present investigation, extracts of Erigeron floribundus leaves obtained by four isolation procedures, including homogenizer-assisted extraction, maceration, sonication and Soxhlet extraction, were compared for their qualitative and quantitative phytochemical composition as well as for their antioxidant and enzyme inhibitory activities. The total phenolic, phenolic acid and flavanol contents were highest in homogenizer-assisted extraction, while maceration contained slightly higher total flavanol content. High-performance liquid chromatography – mass spectrometry (HPLC-DAD-MSn) analysis revealed that the hydroxycinnamic esters of quinic acids were the most abundant constituents in the isolates. The results showed that the extracts are promising sources of antioxidants with the homogenizer-assisted approach the most suitable. The extracts displayed notable enzyme inhibitory effects against acetylcholinesterases (7.98 ± 0.12 to 8.40 ± 0.09 mg galatamine equivalents (GALAE)/g extract) and tyrosinase (122.73 ± 0.43 to 111.36 ± 1.65 mg kojic acid equivalents (KAE)/g extract). In conclusion, the results demonstrate promising biological activities of E. floribundus isolates obtained via innovative extraction techniques, thereby offering preliminary information for the validation of the traditional medicinal uses of this plant.

中文翻译：

---

通过四种分离程序获得的 Erigeron Floribundus (Kunth) Sch.Bip 提取物的化学和生物学表征

摘要 在本研究中，比较了通过均质机辅助提取、浸渍、超声处理和索氏提取四种分离方法获得的绣线菊叶提取物的定性和定量植物化学成分以及抗氧化和酶抑制活性。在均质机辅助提取中，总酚、酚酸和黄烷醇含量最高，而浸渍中总黄烷醇含量略高。高效液相色谱-质谱 (HPLC-DAD-MSn) 分析表明，奎宁酸的羟基肉桂酸酯是分离物中最丰富的成分。结果表明，这些提取物是有希望的抗氧化剂来源，其中均质机辅助方法最合适。提取物对乙酰胆碱酯酶（7.98 ± 0.12 至 8.40 ± 0.09 毫克加拉胺当量（GALAE）/g 提取物）和酪氨酸酶（122.73 ± 0.43 至 111.36 ± 1.65 毫克曲酸提取物）显示出显着的酶抑制作用。总之，结果证明了通过创新提取技术获得的 E. floribundus 分离株具有良好的生物活性，从而为验证该植物的传统药用用途提供了初步信息。