An analytical procedure has been introduced to enable a study of the excretion of free bisphenol A (BPA), total BPA and its main metabolite bisphenol A glucuronide (BPA–GLUC). In the experiment, in which 100 μg/kg b. w. BPA was administered daily to one Istrian Pramenka sheep for 5 days with consecutive urine and feces samples being taken, BPA and total BPA were determined in samples using high-performance liquid chromatography (HPLC) with fluorescence detection. Because of their good recovery, precision, and sensitivity, the methods have also proved applicable to further ecotoxicological studies of free BPA, BPA–GLUC and total BPA. The results were subsequently compared with reported field studies of BPA in livestock excreta.

引入了一种分析程序来研究游离双酚A（BPA），总BPA及其主要代谢物双酚A葡萄糖醛酸（BPA–GLUC）的排泄。在该实验中，每天向一只伊斯特拉普拉门卡羊施用100μg/ kg体重的BPA，持续5天，连续采集尿液和粪便样品，然后使用高效液相色谱（HPLC）测定样品中的BPA和总BPA。荧光检测。由于其良好的回收率，精密度和灵敏性，这些方法也被证明可用于游离BPA，BPA-GLUC和总BPA的进一步生态毒理学研究。随后将结果与家畜粪便中双酚A的现场报道进行了比较。