An original voltammetric screening method, employing glassy carbon electrode (GCE) with the differential-pulse voltammetry technique (DPV), has been developed to determine residues of the anti-parasitic agent Ronidazole (RNZ) in bovine meat. By using cyclic voltammetry (CV), it has been demonstrated that an irreversible cathodic process occurs at approximately −0.740 V (vs. Ag|AgCl, KCl 3 mol L⁻¹) in a 0.100 mol L⁻¹ phosphate buffer at pH 6.5 as supporting electrolyte. Furthermore, the behavior of RNZ in CV indicates the occurrence of a diffusion mass transfer process to the working electrode surface. The RNZ reduction mechanism was proposed as a 6-electron transfer, similar to Metronidazole under the same pH range. Quantification of RNZ and method validation were then carried out by DPV. The relative standard deviation (RSD) were 3.21% for intraday precision of 10 consecutive repetitions and 6.78% for interday precision after five analysis. Limits of detection and quantification were also obtained, and the values were 0.107 and 0.358 mg kg⁻¹, respectively. The recovery percentage for three different concentrations of RNZ in the bovine meat matrix ranged between 98.1% and 100.3%. The method proved to be efficient for screening RNZ in bovine meat.

已经开发出一种原始的伏安法筛选方法，该方法采用玻璃碳电极（GCE）和差分脉冲伏安法（DPV）来确定牛肉中抗寄生虫剂Ronidazole（RNZ）的残留量。通过使用循环伏安法（CV），已证明在0.100 mol L ^-1中约-0.740 V（vs。Ag | AgCl，KCl 3 mol L ^-1）发生不可逆的阴极过程。pH 6.5的磷酸盐缓冲液作为支持电解质。此外，RNZ在CV中的行为表明发生了向工作电极表面的扩散传质过程。提出了RNZ还原机制为6电子转移，类似于在相同pH范围内的甲硝唑。然后通过DPV进行RNZ的定量和方法验证。经过5次分析后，连续10次重复的日内精度的相对标准偏差（RSD）为3.21％，日间精度为6.78％。还获得了检出限和定量限，分别为0.107和0.358 mg kg ^-1， 分别。牛肉基质中三种不同浓度的RNZ的回收率在98.1％至100.3％之间。事实证明该方法可有效筛查牛肉中的RNZ。