The effect of E93 knockdown on female reproduction in the red flour beetle, Tribolium castaneum.,Archives of Insect Biochemistry and Physiology

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The effect of E93 knockdown on female reproduction in the red flour beetle, Tribolium castaneum.
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-05-11 , DOI: 10.1002/arch.21688
Duaa Musleh Eid ₁ , Shankar C R R Chereddy ₁ , Subba Reddy Palli ₁

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The E93 transcription factor is a member of helix‐turn‐helix transcription factor family containing a Pip‐squeak motif. This ecdysone primary response gene was identified as a regulator of cell death in Drosophila melanogaster where it is involved in ecdysone‐induced autophagy and caspase activity that mediate degeneration of larval tissues during metamorphosis from larva to pupa. However, its function in adult insects is not well studied. To study E93 function in the red flour beetle, Tribolium castaneum, double‐stranded RNA (dsRNA) targeting E93 (dsE93) was injected into newly emerged adults. Knockdown of E93 caused a decrease in the synthesis of vitellogenin (Vg), oocyte development, and egg‐laying. Sequencing of RNA isolated from adults injected with dsE93 and control dsmalE (dsRNA targeting Escherichia coli malE gene) followed by differential gene expression analysis showed upregulation of genes involved in the metabolism of reserved nutrients. E93 knockdown induced changes in gene expression resulted in a decrease in Vg synthesis in the fat body and oocyte maturation in ovaries. Mating experiments showed that females injected with dsE93 did not lay eggs. Knockdown of E93 caused a reduction in the number and size of lipid droplets in the fat body when compared with that in control beetles injected with dsmalE. These data suggest that during the first 2–3 days after the emergence of adult females, E93 suppresses genes coding for enzymes that metabolize reserved nutrients until initiation of vitellogenesis and oogenesis.

中文翻译：

E93 敲低对红面粉甲虫 Tribolium castaneum 雌性繁殖的影响。

E93 转录因子是包含 Pip-squeak 基序的 helix-turn-helix 转录因子家族的成员。这种蜕皮激素初级反应基因被鉴定为果蝇细胞死亡的调节因子，它参与蜕皮激素诱导的自噬和半胱天冬酶活性，介导幼虫组织在从幼虫到蛹的变态过程中发生退化。然而，它在成年昆虫中的功能还没有得到很好的研究。为了研究 E93 在赤拟谷盗中的功能，将靶向 E93 (dsE93) 的双链 RNA (dsRNA) 注射到新出现的成虫中。E93 的敲低导致卵黄蛋白原 (Vg) 合成、卵母细胞发育和产卵减少。从注射了 dsE93 和对照的成人中分离出的 RNA 的测序dsmalE（靶向大肠杆菌 malE基因的 dsRNA）随后进行的差异基因表达分析显示，参与保留营养素代谢的基因上调。E93敲低诱导的基因表达变化导致脂肪体中 Vg 合成减少和卵巢中卵母细胞成熟。交配实验表明注射了 dsE93 的雌性不产卵。与注射dsmalE的对照甲虫相比，E93的敲低导致脂肪体中脂滴的数量和大小减少. 这些数据表明，在成年雌性出现后的前 2-3 天内，E93 抑制编码酶的基因，这些酶代谢保留的营养物质，直到开始卵黄发生和卵子发生。

更新日期：2020-05-11

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