WRKY transcription factors WRKY12 and WRKY13 interact with SPL10 to modulate age-mediated flowering.,Journal of Integrative Plant Biology

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WRKY transcription factors WRKY12 and WRKY13 interact with SPL10 to modulate age-mediated flowering.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-05-12 , DOI: 10.1111/jipb.12946
Zhenbing Ma _{1,

2} , Wei Li ₃ , Houping Wang _{2,

4} , Diqiu Yu _{2,

4}

Affiliation

WRKY12 and WRKY13 are two WRKY transcription factors that play important roles in the control of flowering time under short‐day (SD) conditions. The temporally regulated expression of WRKY12 and WRKY13 indicates that they may be involved in the age‐mediated flowering pathway. However, their roles in this pathway are poorly understood. Here, we show that the transcription of WRKY12 and WRKY13 is directly regulated by SQUAMOSA PROMOTER BINDING–LIKE 10 (SPL10), a transcription factor downstream of the age pathway. Binding and activation analyses revealed that SPL10 functions as a positive regulator of WRKY12 and a negative regulator of WRKY13. Further mechanistic investigation revealed that WRKY12 and WRKY13 physically interact with SPL10 and that both of them bind to the promoter of miR172b. Thus, the WRKY12‐SPL10 and WRKY13‐SPL10 interactions facilitate and inhibit SPL10 transcriptional function, respectively, to regulate miR172b expression. Together, our results show that WRKY12 and WRKY13 participate in the control of age‐mediated flowering under SD conditions though physically interacting with SPLs and co‐regulating the target gene miR172b.

中文翻译：

WRKY转录因子WRKY12和WRKY13与SPL10相互作用以调节年龄介导的开花。

WRKY12和WRKY13是两个WRKY转录因子，它们在短日（SD）条件下对开花时间的控制中起着重要作用。WRKY12和WRKY13的时间调控表达表明它们可能参与了年龄介导的开花途径。但是，人们对它们在该途径中的作用了解甚少。在这里，我们显示WRKY12和WRKY13的转录直接受SQUAMOSA PROMOTER BINDING-Like 10（SPL10）（年龄途径下游的转录因子）调控。结合和激活分析显示，SPL10充当WRKY12的正调节剂和WRKY13的负调节剂。另外机械调查显示，WRKY12和WRKY13物理相互作用与SPL10和两者结合的启动子miR172b。因此，WRKY12-SPL10和WRKY13-SPL10的相互作用分别促进和抑制SPL10转录功能，从而调节miR172b的表达。总之，我们的结果表明WRKY12和WRKY13通过与SPL物理相互作用并共同调控靶基因miR172b参与了SD条件下年龄介导的开花的控制。

更新日期：2020-05-12

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