FHA2 is a plant-specific ISWI subunit responsible for stamen development and plant fertility.,Journal of Integrative Plant Biology

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FHA2 is a plant-specific ISWI subunit responsible for stamen development and plant fertility.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-05-12 , DOI: 10.1111/jipb.12945
Bo-Wen Gu _{1,

2} , Lian-Mei Tan ₂ , Cui-Jun Zhang ₂ , Xiao-Mei Hou ₂ , Xue-Wei Cai ₂ , She Chen _{2,

3} , Xin-Jian He _{2,

3}

Affiliation

Imitation Switch (ISWI) chromatin remodelers are known to function in diverse multi‐subunit complexes in yeast and animals. However, the constitution and function of ISWI complexes in Arabidopsis thaliana remain unclear. In this study, we identified forkhead‐associated domain 2 (FHA2) as a plant‐specific subunit of an ISWI chromatin‐remodeling complex in Arabidopsis. By in vivo and in vitro analyses, we demonstrated that FHA2 directly binds to RLT1 and RLT2, two redundant subunits of the ISWI complex in Arabidopsis. The stamen filament is shorter in the fha2 and rlt1/2 mutants than in the wild type, whereas their pistil lengths are comparable. The shorter filament, which is due to reduced cell size, results in insufficient pollination and reduced fertility. The rlt1/2 mutant shows an early‐flowering phenotype, whereas the phenotype is not shared by the fha2 mutant. Consistent with the functional specificity of FHA2, our RNA‐seq analysis indicated that the fha2 mutant affects a subset of RLT1/2‐regulated genes that does not include genes involved in the regulation of flowering time. This study demonstrates that FHA2 functions as a previously uncharacterized subunit of the Arabidopsis ISWI complex and is exclusively involved in regulating stamen development and plant fertility.

中文翻译：

FHA2是植物特定的ISWI亚基，负责雄蕊发育和植物育性。

已知模仿开关（ISWI）染色质重塑剂可在酵母和动物中的多种多亚基复合物中发挥作用。然而，在拟南芥中ISWI复合物的组成和功能仍不清楚。在这项研究中，我们确定了叉头相关结构域2（FHA2）是拟南芥ISWI染色质重塑复合体的植物特异性亚基。通过体内和体外分析，我们证明FHA2直接结合RLT1和RLT2，这是拟南芥ISWI复合物中的两个冗余亚基。雄蕊花丝在fha2和rlt1 / 2中较短突变体比在野生型，而它们的雌蕊长度是可比的。较短的长丝是由于细胞大小减小导致授粉不足和繁殖力降低。所述rlt1 / 2突变体显示了早期开花表型，而表型不被共享fha2突变体。与FHA2的功能特异性一致，我们的RNA序列分析表明fha2突变体影响RLT1 / 2调控基因的一个子集，其中不包括参与开花时间调控的基因。这项研究表明FHA2充当拟南芥ISWI复合物以前未表征的亚基，并且仅参与调节雄蕊发育和植物育性。

更新日期：2020-05-12

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