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Proteomic analysis of "Oriental Beauty" oolong tea leaves with different degrees of leafhopper infestation.
Rapid Communications in Mass Spectrometry ( IF 1.8 ) Pub Date : 2020-05-12 , DOI: 10.1002/rcm.8825 Han-Ju Chien,Man-Miao Yang,Wei-Chen Wang,Xiang-Gui Hong,Yi-Feng Zheng,Jie-Teng Toh,Chia-Chang Wu,Chien-Chen Lai
Rapid Communications in Mass Spectrometry ( IF 1.8 ) Pub Date : 2020-05-12 , DOI: 10.1002/rcm.8825 Han-Ju Chien,Man-Miao Yang,Wei-Chen Wang,Xiang-Gui Hong,Yi-Feng Zheng,Jie-Teng Toh,Chia-Chang Wu,Chien-Chen Lai
RATIONALE
Oriental Beauty, a variety of oolong tea native to Taiwan, is highly prized by connoisseurs for its unique fruity aroma and sweet taste. Leaves of Oriental Beauty vary in appearance, aroma and taste depending on the degree of tea green leafhopper (Jacobiasca formosana) infestation. In this study, the aim is to investigate the differential expression of proteins in leaves with low, medium and high degrees of leafhopper infestation.
METHODS
Proteomic techniques 2DE (2-dimensional electrophoresis) and nanoscale LC/MS/MS (nano-LC/MS/MS) were used to investigate the differential expression of proteins in tea leaves with different degrees of leafhopper infestation.
RESULTS
A total of 89 proteins were found to exhibit significant differences in expression. In a gene ontology analysis, it was found that most of these proteins participate in biosynthesis, carbohydrate metabolism, transport, responses to stress and amino acid metabolism.
CONCLUSIONS
These results indicated that the unique aroma and taste of the leaves might be influenced by their protein expression profiles, as well as related factors such as defensive responses to tea green leafhopper saliva.
更新日期:2020-05-12