Through a combination of synthetic and analytical chemistry, the structure of the modified nucleoside 4‐methylthiouridine was confirmed in bacterial tRNA. The modification is of low abundance and forms after bacterial stress induced by methylating agents; this argues for its nature as a natural tRNA lesion. By pulse chase analysis, we show its repair through a dethiomethylation mechanism in vivo.

中文翻译：

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细菌tRNA中4-甲基硫尿苷的合成及代谢命运。

通过合成和分析化学的结合，在细菌tRNA中证实了修饰的核苷4-甲基硫尿苷的结构。修饰的丰度低，在甲基化剂诱导细菌胁迫后形成。这证明了其作为天然tRNA病变的性质。通过脉冲追踪分析，我们通过体内的脱硫甲基化机制显示了其修复作用。