To facilitate the early diagnosis of Alzheimer's disease and mild cognitive impairment patients, we developed a cantilever‐based microsensor that immobilized liposomes of various phospholipids to detect a trace amount of amyloid β (Aβ) protein, and investigated its aggregation and fibrillization on model cell membranes in human serum. Three species of liposomes composed of different phospholipids of 1,2‐dipalmtoyl‐sn‐glycero‐3‐phosphocholine (DPPC), DPPC/phosphatidyl ethanolamine and 1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphorylglycerol having varied hydrophilic groups were applied, which showed different chronological interactions with Aβ(1–40) protein and varied sensitivities of the cantilever sensor, depending on their specific electrostatic charged conditions, hydrophilicity, and membrane fluidity. 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine (DMPC) having short hydrophobic carbon chains confirmed to show a large interaction with Aβ(1–40) and a high sensitivity. Furthermore, the incorporation of cholesterol into DMPC was effective to selectively detect Aβ(1–40) in human serum, which effect was also checked by quartz crystal microbalance. Finally, Aβ detection of 100‐pM order was expected selectively in the serum by using the developed biosensor.

中文翻译：

---

通过固定掺入胆固醇的脂质体的微悬臂梁传感器无标记、按时间顺序和选择性检测血清中淀粉样蛋白 β 蛋白的聚集和纤维化。

为了促进阿尔茨海默病和轻度认知障碍患者的早期诊断，我们开发了一种基于悬臂梁的微传感器，该微传感器固定了各种磷脂的脂质体，以检测微量的淀粉样β (Aβ) 蛋白，并研究其在模型细胞膜上的聚集和纤维化在人血清中。三种脂质体的1,2- dipalmtoyl-的不同的磷脂组成的SN -glycero -3-磷酸胆碱（DPPC），DPPC /磷脂酰乙醇胺和1,2- dipalmitoyl- SN应用了具有不同亲水基团的-glycero-3-phosphorylglycerol，显示出与Aβ(1-40)蛋白的不同时间相互作用和悬臂传感器的不同灵敏度，这取决于它们特定的静电荷条件、亲水性和膜流动性。具有短疏水碳链的1,2-二肉豆蔻酰基-sn-甘油-3-磷酸胆碱 (DMPC) 被证实与 Aβ(1-40) 有很大的相互作用和高灵敏度。此外，将胆固醇掺入 DMPC 可以有效地选择性检测人血清中的 Aβ(1-40)，石英晶体微量天平也检查了这种效果。最后，通过使用开发的生物传感器，预期在血清中选择性检测 100-pM 级的 Aβ。