The common practice of therapeutic drug monitoring (TDM) involves the quantification of drug plasma concentrations at a specific time in a dosing window. Although TDM for antibiotics is not considered mandatory, it may represent a valid tool for clinicians in order to limit antibiotic resistance and avoid therapeutic failures. The aim of our study was to develop and validate a high‐performance liquid chromatography–diode array detection method for simultaneous quantification of 10 antibiotics in plasma. This method has a fast analytical procedure that uses the same chromatographic conditions to quantify ceftazidime, ceftriaxone, meropenem, ertapenem, ciprofloxacin, tigecycline, ampicillin, levofloxacin and piperacillin, plus the β‐lactamase inhibitor tazobactam. Method validation was ensured by testing selectivity, accuracy, precision, limits of detection and quantification, recovery and stability. The calibration ranges, established accordingly to the expected plasma concentration in patients, showed a coefficient of determination >0.996 for all compounds. Within‐ and between‐days precisions reported a coefficient of variation >15%. Similarly, the accuracy evaluation reported a relative standard deviation of <10% for each antibiotic. The recovery ranged between 97 and 103% for all compounds. This method could represent a useful tool for TDM of antibiotics.

中文翻译：

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一种新的HPLC-DAD方法，可对10种抗生素进行当代定量，以监测重症儿科患者的治疗药物。

治疗药物监测（TDM）的常规做法是在给药窗口中特定时间对药物血浆浓度进行定量。尽管抗生素的TDM并不是强制性的，但它可能代表临床医生有效的工具，以限制抗生素耐药性并避免治疗失败。我们研究的目的是开发和验证一种高效液相色谱-二极管阵列检测方法，用于同时定量测定血浆中的10种抗生素。该方法具有快速的分析程序，使用相同的色谱条件对头孢他啶，头孢曲松，美洛培南，厄他培南，环丙沙星，替加环素，氨苄青霉素，左氧氟沙星和哌拉西林以及β进行定量。内酰胺酶抑制剂他唑巴坦。通过测试选择性，准确性，精密度，检测和定量限，回收率和稳定性来确保方法验证。根据患者的预期血浆浓度确定的校准范围显示所有化合物的测定系数均> 0.996。日内和日间精度报告的变异系数> 15％。同样，准确性评估报告每种抗生素的相对标准偏差<10％。所有化合物的回收率在97％至103％之间。该方法可能代表了用于TDM抗生素的有用工具。