Broad and potent human monoclonal neutralizing antibodies have considerable potential in the prevention and treatment of acquired immunodeficiency syndrome (AIDS). To identify the key amino acid recognition site contacted with neutralizing antibody Y498, peptides were panned from the PhD-12 peptide library and predicted using online software. Then, four key amino acid sites, G367, D368, E370, and V372 located on the CD4 binding loop on gp120 of envelope of human immunodeficiency virus-1 (HIV-1), were found to determine the neutralization of antibody Y498. Residue E370 is in the deep part of the CD4 binding loop, which affects Y498-mediated neutralization. This form of recognition leads to a somewhat limiting neutralization spectrum of neutralizing antibody Y498, although it has some neutralization ability. Further study of the interactions between the neutralizing antibody Y498 and its epitope on the surface of the virus may facilitate vaccine development and so prevent new AIDS cases.

中文翻译：

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CD4 结合环负责中和人单克隆中和抗体 Y498。

广泛而有效的人单克隆中和抗体在预防和治疗获得性免疫缺陷综合征 (AIDS) 方面具有相当大的潜力。为了鉴定与中和抗体 Y498 接触的关键氨基酸识别位点，从 Phd-12 肽库中筛选出肽并使用在线软件进行预测。然后，发现位于人类免疫缺陷病毒-1 (HIV-1) 包膜 gp120 上 CD4 结合环上的四个关键氨基酸位点 G367、D368、E370 和 V372，以确定抗体 Y498 的中和作用。残基 E370 位于 CD4 结合环的深部，影响 Y498 介导的中和。这种形式的识别导致中和抗体 Y498 的中和谱有限，尽管它具有一些中和能力。