In the present study, the sulfated polysaccharides obtained from Codium fragile (CFCE-PS) were prepared by enzymatic digestion and ethanol precipitation, and the antioxidant properties of CFCE-PS were evaluated using both in vitro and in vivo models. CFCE-PS contained 76.84% sulfated polysaccharides, which comprised 55.78 ± 3.00% of carbohydrate and 21.06 ± 1.07% of sulfate. CFCE-PS scavenged hydroxyl, alkyl, and DPPH radicals with IC₅₀ values of 1.21 ± 0.01, 1.42 ± 0.17, and 6.14 ± 0.78 mg/mL, respectively. CFCE-PS remarkably protected Vero cells against hydrogen peroxide (H₂O₂)-induced damage by reducing the intracellular ROS level, increasing cell viability, and suppressing apoptosis in a dose-dependent manner. Furthermore, CFCE-PS significantly attenuated H₂O₂-mediated oxidative stress in vivo in zebrafish. CFCE-PS dose-dependently improved the survival rate and normalized the heartbeat, as well as reduced ROS, cell death, and lipid peroxidation in H₂O₂-stimulated zebrafish. Taken together, these results demonstrated that CFCE-PS possesses strong in vitro and in vivo antioxidant activities, and it could be used as an ingredient in the functional food, pharmacy, and cosmetic industries.

在本研究中，通过酶消化和乙醇沉淀法制备了从脆弱钴酸钠（CFCE-PS）获得的硫酸化多糖，并使用体内和体外模型评估了CFCE-PS的抗氧化性能。CFCE-PS包含76.84％的硫酸化多糖，其中包含55.78±3.00％的碳水化合物和21.06±1.07％的硫酸盐。CFCE-PS清除的羟基，烷基和DPPH自由基的IC ₅₀值分别为1.21± 0.01、1.42 ±0.17和6.14±0.78 mg / mL。CFCE-PS显着保护Vero细胞免受过氧化氢（H ₂ O ₂降低细胞内ROS水平，增加细胞生存力并以剂量依赖的方式抑制细胞凋亡，从而引起细胞损伤。此外，CFCE-PS在斑马鱼体内显着减轻了H ₂ O ₂介导的氧化应激。CFCE-PS剂量依赖性地提高了H ₂ O ₂刺激的斑马鱼的存活率并使心跳正常化，并降低了ROS，细胞死亡和脂质过氧化。综上所述，这些结果表明CFCE-PS具有强大的体外和体内抗氧化活性，并且可以在功能性食品，制药和化妆品工业中用作成分。