Construction of scaffolds which are similar to natural niches regarding both biochemical composition and mechanical characteristics has gained great attention in the field of tissue engineering. However, application of natural polymers, such as hyaluronic acid, is challenging in construction of scaffolds due to physicochemical properties, difficult to use in electrospinning and low cell adhesion rate. In this study, HA was acetylated to make it soluble in high polarity solvent and blended with PCL for construction of nanofibrous composite (ac-HA/PCL) scaffolds. Chondroinductivity of the constructed scaffolds was investigated using human mesenchymal stem cells (hADSCs). The presence of acetyl groups, as well as morphology and biocompatibility of the composite scaffolds were characterized by HNMR, FTIR, SEM and MTT assay respectively. Expression of cartilage-specific genes (SOX9, Col II and Aggrecan) was monitored by Real-time PCR. Significant increase in expression of Sox9 and Col II as the markers of chondrogenic differentiation as well as the results of Alcian blue staining, indicated the chondro-inductive potential of HA/PCL nanofibrous scaffolds. Acetylated HA was biocompatible with chondroinductivity features, therefore it not only had the positive characteristics of natural HA, but also enhanced the cellular attachment and application potential.

就生物化学组成和机械特性而言，类似于天然壁ni的支架的构造已在组织工程领域引起了极大的关注。然而，由于物理化学性质，难以在电纺中使用以及细胞粘附率低，天然聚合物如透明质酸的应用在支架的构造中具有挑战性。在这项研究中，将HA乙酰化以使其可溶于高极性溶剂中，并与PCL混合以构建纳米纤维复合材料（ac-HA / PCL）支架。使用人间充质干细胞（hADSCs）研究了构建的支架的软骨诱导性。分别通过1 HNMR，FTIR，SEM和MTT分析表征了乙酰基的存在，以及复合支架的形态和生物相容性。通过实时PCR监测软骨特异性基因（SOX9，Col II和Aggrecan）的表达。Sox9和Col II的表达显着增加，作为软骨分化的标志物以及Alcian蓝染色的结果表明，HA / PCL纳米纤维支架具有软骨诱导潜力。乙酰化的HA具有软骨诱导功能的生物相容性，因此它不仅具有天然HA的积极特性，而且还增强了细胞附着和应用潜力。