Identification of antifungal organic compound from B. velezensis AR1 culture supernatant was performed using high-performance liquid chromatography (HPLC) and subjected to proton (1H) and carbon (13C) nuclear magnetic resonance (NMR). From the analysis of the heteronuclear multiple bond correlation, the purified compound was identified as monomethyl succinate (MMSucc). The subsequent investigation of its antifungal activity demonstrated growth inhibition of 5.73 to 100%, 8.75 to 96.67%, and 7.17 to 71.3% on Rhizoctonia solani, Sclerotinia sclerotiorum and Botrytis cinerea at 25 and 550 µg mL− 1 MMSucc, respectively. The minimum inhibitory concentration was 550, 650 and 675 µg mL− 1 for R. solani, S. sclerotiorum and B. cinerea, respectively. Therefore, based on its broad antifungal activities, it is possible to suggest the compound as an alternative source of fungicide.

中文翻译：

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贝雷兹芽孢杆菌 AR1 琥珀酸甲酯的鉴定与表征

使用高效液相色谱 (HPLC) 并进行质子 (1H) 和碳 (13C) 核磁共振 (NMR) 从 B. velezensis AR1 培养上清液中鉴定抗真菌有机化合物。根据异核多键相关性分析，纯化的化合物被鉴定为琥珀酸单甲酯 (MMSucc)。随后对其抗真菌活性的研究表明，在 25 和 550 µg/mL-1 下，对立枯丝核菌、核盘菌和灰葡萄孢菌的生长抑制分别为 5.73% 至 100%、8.75% 至 96.67% 和 7.17% 至 71.3%。R. solani、S. sclerotiorum 和 B. cinerea 的最小抑制浓度分别为 550、650 和 675 µg mL-1。因此，基于其广泛的抗真菌活性，