The production of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) concentrate from cobia liver oil by acetone fractionation of fatty acid salts was investigated in this study. A three-level-three-factor Box-Behnken design was used to evaluate the effects of reaction time, amount of NaOH added, and acetone ratio on the responses (DHA and EPA content and recovery). The results showed that the amount of NaOH added was the most important factor in the process. The DHA content showed an inverse relation with EPA content and recovery, whereas its content increased proportionally with the amount of NaOH added. With a reaction time of 1.51 h, amount of NaOH added at 0.65 times the molar equivalent of free fatty acid (FFA), and acetone ratio at 13.92, a maximum recovery of DHA + EPA was 98.14%, and the obtained concentrate contained 71.23% DHA + EPA. Finally, the lipase-catalyzed esterification of the DHA + EPA concentrate with glycerol was carried out. The acetone fractionation of fatty acid salts is an efficient technique for producing DHA +EPA concentrate. The DHA +EPA concentrate can be used as starting materials for the production of functional lipids to provide n-3 polyunsaturated fatty acids to the consumers.

中文翻译：

---

通过丙酮分馏脂肪酸盐从军曹鱼肝油中浓缩二十二碳六烯酸和二十碳五烯酸。

在这项研究中，通过丙酮分馏脂肪酸盐，从军曹鱼肝油中生产二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）浓缩物。三级三因素Box-Behnken设计用于评估反应时间，添加的NaOH量和丙酮比例对反应（DHA和EPA含量及回收率）的影响。结果表明，NaOH的添加量是该过程中最重要的因素。DHA含量与EPA含量和回收率呈反比关系，而其含量则与NaOH的添加成比例地增加。反应时间为1.51h，NaOH的添加量为游离脂肪酸（FFA）摩尔当量的0.65倍，丙酮比例为13.92，DHA + EPA的最大回收率为98.14％，所得浓缩物含有71。23％DHA + EPA。最后，用甘油将DHA + EPA浓缩物进行脂肪酶催化的酯化反应。脂肪酸盐的丙酮分馏是生产DHA + EPA浓缩物的有效技术。DHA + EPA浓缩物可用作生产功能性脂质的原料，为消费者提供n-3多不饱和脂肪酸。