Solid-state fermentation, featured by water-saving, eco-friendly and high concentration product, is a promising technology in lignocellulosic ethanol industry. However, in solid-state fermentation system, large gas content inside the substrate directly leads to high oxygen partial pressure and inhibits ethanol fermentation. Z. mobilis can produce ethanol from glucose near the theoretical maximum value, but this ethanol yield would be greatly decreased by high oxygen partial pressure during solid-state fermentation. In this study, we applied N2 periodic pulsation process intensification (NPPPI) to ethanol solid-state fermentation, which displaced air with N2 and provided a proper anaerobic environment for Z. mobilis. Based on the water state distribution, the promotion effects of NPPPI on low solid loading and solid-state fermentation were analyzed to confirm the different degrees of oxygen inhibition in ethanol solid-state fermentation. During the simultaneous saccharification solid-state fermentation, the NPPPI group achieved 45.29% ethanol yield improvement and 30.38% concentration improvement compared with the control group. NPPPI also effectively decreased 58.47% of glycerol and 84.24% of acetic acid production and increased the biomass of Z. mobilis. By coupling the peristaltic enzymatic hydrolysis and fed-batch culture, NPPPI made the ethanol yield and concentration reach 80.11% and 55.06 g/L, respectively, in solid-state fermentation.

中文翻译：

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使用运动发酵单胞菌通过N2周期性脉动过程强化对蒸汽爆破玉米秸秆进行固态发酵而获得的高产乙醇。

固态发酵具有节水，环保和高浓度的特点，是木质纤维素乙醇工业中的一项有前途的技术。然而，在固态发酵系统中，底物中的大量气体直接导致高的氧分压并抑制乙醇发酵。运动发酵单胞菌可以从接近理论最大值的葡萄糖中产生乙醇，但是在固态发酵过程中，高氧气分压会大大降低乙醇产量。在这项研究中，我们将N2周期性脉动过程增强（NPPPI）应用于乙醇固态发酵，从而用N2置换了空气，并为运动发酵单胞菌提供了合适的厌氧环境。根据水的状态分布，分析了NPPPI对低固形物和固态发酵的促进作用，以证实乙醇固态发酵中不同程度的氧抑制。在同步糖化固态发酵过程中，与对照组相比，NPPPI组的乙醇收率提高了45.29％，浓度提高了30.38％。NPPPI还有效降低了58.47％的甘油和84.24％的乙酸产量，并增加了运动发酵单胞菌的生物量。通过将蠕动酶水解和补料分批培养相结合，NPPPI在固态发酵中使乙醇的收率和浓度分别达到80.11％和55.06 g / L。与对照组相比，NPPPI组的乙醇收率提高了45.29％，浓度提高了30.38％。NPPPI还有效降低了58.47％的甘油和84.24％的乙酸产量，并增加了运动发酵单胞菌的生物量。通过将蠕动酶水解和补料分批培养相结合，NPPPI在固态发酵中使乙醇的收率和浓度分别达到80.11％和55.06 g / L。与对照组相比，NPPPI组的乙醇收率提高了45.29％，浓度提高了30.38％。NPPPI还有效降低了58.47％的甘油和84.24％的乙酸产量，并增加了运动发酵单胞菌的生物量。通过将蠕动酶水解和补料分批培养相结合，NPPPI在固态发酵中使乙醇的收率和浓度分别达到80.11％和55.06 g / L。