Land plants exist in close association with bacterial and fungal microbes, where some associations can be pathogenic and others can be mutualistic/beneficial. One such relation exists between host plant, Cuminum cyminum L. (Cumin) and Fusarium oxysporum f. sp. cumini (Foc), the causal pathogen of cumin wilt and Pseudomonas fluorescens (Pf), where Pf acts as a bio-agent for inhibiting Foc and promoting plant growth of cumin. In this study, antagonism by 10 different Pf isolates against Foc was studied under laboratory conditions through percent growth inhibition and biochemical mechanisms. Among these Pf isolates, Pf-5 exhibited the highest in vitro growth inhibition (82.51%). A positive correlation was observed between percent growth inhibition and specific activities of hydrolytic enzymes, chitinase, β-1, 3 glucanase, and protease, where a negative correlation was observed with cell wall degrading enzymes, cellulase and polygalacturonase. To conclude, isolate Pf-5 could be a potential biocontrol agent for Fusarium wilt disease of cumin.

中文翻译：

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在体外筛选不同的荧光假单胞菌菌株以研究尖孢镰刀菌拮抗过程中裂解酶的产生和生长抑制。sp。蒲桃，必造成孜然的病原体

陆地植物与细菌和真菌微生物密切相关，其中一些可能是致病性的，而另一些则可能是互利的/有益的。寄主植物小茴香（Cuminum cyminum L.）（Cumin）和尖孢镰刀菌（Fusarium oxysporum f。sp。cumini（Foc），小茴香枯萎病和荧光假单胞菌（Pf）的病原体，其中Pf用作抑制Foc和促进小茴香植物生长的生物制剂。在这项研究中，在实验室条件下，通过百分比生长抑制和生化机制研究了10种不同的Pf分离株对Foc的拮抗作用。在这些Pf分离物中，Pf-5表现出最高的体外生长抑制（82.51％）。观察到生长抑制百分比与水解酶，几丁质酶，β-1、3葡聚糖酶和蛋白酶的比活性之间呈正相关，与细胞壁降解酶，纤维素酶和聚半乳糖醛酸酶呈负相关。总之，分离株Pf-5可能是小茴香枯萎病的潜在生物防治剂。