Abstract

The Mi gene is known to involve in the resistance response to some Root knot nematodes, Meloidogyne spp. in tomato plants Lycopersicon esculentum. Root knot nematodes cause significant damages to almost all crops in the world. Among Meloidogyne species, Meloidogyne incognita is one of the most damages species causing a vast amount of crop loss including tomato plants. The resistance gene may be found in some tomato genotypes or cultivars. Determining the resistance gene, Mi, in tomato cultivars is very important to control root knot nematodes. For this purpose, this study was conducted in 99 tomato genotypes that were screened for resistance against Meloidogyne incognita in molecular and screening assays. Results revealed that Mi gene was only determined in one genotype (Tom113). The result of the screening assay indicated that one of the genotypes (Tom113) showed an immune reaction against the nematode; two genotypes (Tom146, Tom141) were found intermediate; 17 genotypes moderately susceptible, 43 genotypes susceptible and 36 genotypes found highly susceptible. Results of the screening assay confirmed the presence of Mi gene in a tomato genotype, which accompanied with a decreased ability for the nematode in causing the infection. Immune and some promising genotypes are thought as important genetic tools for breeding studies for future works.

中文翻译：

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Mi基因和线虫抗性对番茄基因型的影响

摘要

所述祢基因是已知的电阻响应于一些根结线虫，涉及根结线虫属。在番茄植物番茄中的表达。根结线虫对世界上几乎所有农作物造成重大破坏。在根结线虫物种中，根结线虫（Meloidogyne incognita）是造成最多农作物损失（包括番茄植物）的破坏力最强的物种之一。抗性基因可能存在于某些番茄基因型或栽培品种中。确定番茄品种的抗性基因Mi对控制根结线虫非常重要。为此，本研究针对99种番茄基因型进行了筛选，这些基因型针对分子和筛选测定中的根结线虫。结果显示，Mi基因仅在一种基因型（Tom113）中确定。筛选分析的结果表明，其中一种基因型（Tom113）表现出针对线虫的免疫反应。发现两个基因型（Tom146，Tom141）处于中间；中度易感的基因型有17种，易感的基因型有43种，高度易感的基因型有36种。筛选测定的结果证实了番茄基因型中存在Mi基因，其伴随着线虫引起感染的能力降低。免疫和一些有前途的基因型被认为是未来研究育种研究的重要遗传工具。