Abstract

Routine, GTG-, and Ag-staining techniques for the metaphase chromosome analysis were used to determine the spontaneous frequency of chromosomal aberrations and the level of chromosomal variability in the blood lymphocytes of Ukrainian buffaloes (Bubalus bubalis L.). It has been established that the diploid complement of chromosomes within the studied population of animals consists of 50 chromosomes (2n = 50, XX; 2n = 50, XY). Individual chromosomal variability was detected in some animals in the form of cells with aneuploidy and polyploidy sets of chromosomes as well as in the form of structural aberrations in autosomes. In particular, the chromosomal analysis based on differential staining technique has shown a duplicate in one chromosome of the second pair in individual females as well as an X monosomia and a chimerism over sex chromosomes. The total spontaneous level of chromosomal instability in the peripheral blood lymphocytes reached, on average, 14.35 ± 2.03% due to abnormalities of chromosomal and chromatid types. The Ag staining method was used to detect active NORs in six chromosome pairs (3p, 4p, 6q, 21q, 23q, and 24q).

中文翻译：

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乌克兰水牛（Bubalus bubalis L.）体细胞中染色体畸变的频率

摘要

使用常规，GTG和Ag染色技术进行中期染色体分析，以确定乌克兰水牛（Bubalus bubalis L.）的血液中染色体畸变的自发频率和染色体变异水平。已经确定，在所研究的动物种群中，染色体的二倍体补体由50条染色体组成（2 n = 50，XX； 2 n= 50，XY）。在某些动物中，以具有非整倍性和多倍性染色体组的细胞以及常染色体中的结构畸变的形式检测到个体的染色体变异性。尤其是，基于差异染色技术的染色体分析显示，在雌性个体中，第二对染色体的一条染色体中有一条重复，并且在性别染色体上存在X单体和嵌合现象。由于染色体和染色单体类型的异常，外周血淋巴细胞中染色体不稳定的总自发水平平均达到14.35±2.03％。Ag染色法用于检测六对染色体（3p，4p，6q，21q，23q和24q）中的活性NOR。