Abstract

The content of indole-3-acetic (IAA), gibberellic (GA₃), abscisic acids (ABA), and cytokinins—zeatin (Z), zeatin glucoside (ZG), zeatin riboside (ZP), isopentenyladenin (iР), and isopentenyladenosine (iРА)—was determined using the method of high-performance liquid chromatography in fern Polystichum aculeatum gametophytes at different stages of morphogenesis in vitro culture. It was shown that, at the stage of spatulate prothallium development, gametophytes contained significant amounts of GA₃ and IAA, while the content of ABA and cytokinins was low. The inactive ZG and active iP were dominant. At the stage of cordiform thallus formation, the level of GA₃ increased by 1.6, IAA by 2.4, and ABA by 1.7 times. The content of inactive iPA increased twice, significantly increased the level of active ZR and iP. At the development stage of sporophyte on the surface of gametophyte thallus, the levels of IAA, ABA, and GA₃ decreased. The accumulation of inactive ZG and decrease in the content of active zeatin and zeatin riboside were documented. At all stages of gametophytes’ morphogenesis, GA₃ was dominant, and its highest content was detected during the development of archegonia and antheridia. The pattern of gibberellic acid and zeatin riboside accumulation has shown the key role of these hormones in the regulation of growth processes and the development of reproductive structures of P. aculeatum gametophytes.

中文翻译：

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体外培养中不同形态发生阶段的蕨类植物蕨类配子体的内源植物激素

摘要

吲哚-3-乙酸（IAA），赤霉素（GA ₃），脱落酸（ABA）和细胞分裂素的含量-玉米蛋白（Z），玉米蛋白糖苷（ZG），玉米蛋白核糖苷（ZP），异戊烯腺嘌呤（iР）和异戊烯基腺苷（iРА）—是通过高效液相色谱法在体外培养的不同形态发生阶段的蕨类多囊蕨配子体中测定的。结果表明，在成瓣的前角质发育阶段，配子体中含有大量的GA ₃和IAA，而ABA和细胞分裂素的含量较低。无效的ZG和有效的iP占主导地位。在脐带状菌体形成阶段，GA ₃的水平增长了1.6倍，IAA增长了2.4倍，ABA增长了1.7倍。非活性iPA的含量增加了两倍，显着增加了活性ZR和iP的水平。在配子体菌体表面孢子体的发育阶段，IAA，ABA和GA _3的含量下降。记录了无活性ZG的积累以及活性玉米蛋白和玉米蛋白核糖苷含量的降低。在配子体形态形成的所有阶段，GA ₃占主导地位，并且在古菌和花药的发育过程中检测到最高含量。赤霉素和玉米素核糖苷积累的模式表明，这些激素在杆状假单胞菌配子体的生长过程和生殖结构的发育中起着关键作用。