Abstract

The effects of cryopreservation for different periods of time on the amplitudes of AMPA (α‑amino-3-hydroxy-5-methylisoxazole-4-propionic acid) and NMDA (N-methyl-D-aspartate) glutamatergic ionotropic mechanisms and the action potential of the lateral olfactory tract (AP LOT) at –20°C and subsequent warming to 37°C was studied. Experiments were performed on brain slices of 22 nonhibernating animals—Wistar rats. After cryopreservation, the glutamatergic ionotropic mechanisms were preserved and restored using electrophysiological recording of the AMPA and NMDA potentials and the total AP LOT. The AMPA-dependent mechanisms and the activity of conductive LOT fibers were restored to the normothermal values; the recovery of the NMDA-dependent mechanisms was incomplete and averaged 34% compared with the normothermal values; the long-term post-tetanic potentiation was not restored after cryopreservation.

中文翻译：

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冷冻时间对离子型谷氨酸能机制活性的影响：体外研究

摘要

冷冻不同时间段对AMPA（α-氨基-3-羟基-5-甲基异恶唑-4-丙酸）和NMDA（N-甲基-D-天冬氨酸）谷氨酸能离子化机理和嗅觉侧道（AP LOT）在–20°C及随后升温至37°C时的动作电位。实验是对22只非冬眠动物-Wistar大鼠的大脑切片进行的。冷冻保存后，使用AMPA和NMDA电位以及总AP LOT的电生理记录来保存和恢复谷氨酸能离子化机制。AMPA依赖的机制和导电LOT纤维的活性恢复到常温值；NMDA依赖机制的恢复不完全，与常温值相比平均恢复34％；冷冻保存后不能恢复长期的强直性增强作用。