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Identification and pathogenicity of plant viruses infecting Valeriana fauriei in Japan
Journal of General Plant Pathology ( IF 1.0 ) Pub Date : 2020-05-11 , DOI: 10.1007/s10327-020-00925-3
T. Uehara-Ichiki , M. Ohashi , K. Hanada , M. Igarashi , A. Hishida

Samples from Valeriana fauriei Briq. plants were collected from six prefectures to survey viral pathogens. By next-generation sequencing and RT-PCR analysis, asparagus virus 2 (AV-2), broad bean wilt virus 2 (BBWV-2), cucumber mosaic virus (CMV), gaillardia latent virus (GaILV), ligustrum necrotic ringspot virus (LNRSV), and tomato spotted wilt orthotospovirus (TSWV) were detected. Many collected samples were coinfected with 2–4 viruses. V. officinalis was also back-inoculated with one or a mixture of isolated viruses to confirm pathogenicity of isolated viruses. This report is the first of GaILV and LNRSV in Japan and the first of AV-2 on V. fauriei.

中文翻译:

日本感染缬草的植物病毒的鉴定和致病性

Valeriana fauriei Briq的样品。从六个州收集了一些植物以调查病毒病原体。通过下一代测序和RT-PCR分析,芦笋病毒2(AV-2),蚕豆枯萎病毒2(BBWV-2),黄瓜花叶病毒(CMV),盖拉潜在病毒(GaILV),女贞子坏死环斑病毒( LNRSV)和番茄斑点枯萎原病毒(TSWV)被检测到。许多收集的样品都感染了2-4种病毒。还用一种或多种分离病毒的混合物反接种了V. officinalis,以确认分离病毒的致病性。该报告是日本的GaILV和LNRSV的第一个报告,也是关于V. fauriei的AV-2的第一个报告。
更新日期:2020-05-11
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