In 2016, a serious outbreak of apple scab (caused by Venturia inaequalis) in Aomori Prefecture, Japan, resulted from reduced fungal sensitivity to sterol demethylation inhibitors (DMIs). In this study, the lanosterol 14α-demethylase (CYP51A1) gene of V. inaequalis isolates from Aomori Prefecture was analyzed. A nonsynonymous nucleotide polymorphism causing an amino acid substitution from tyrosine to phenylalanine at position 133 (Y133F) in CYP51A1 was closely associated with low sensitivity to DMIs. This is first report of a point mutation in CYP51A1 associated with low sensitivity to DMIs in V. inaequalis.

中文翻译：

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Venturia inaequalis CYP51A1中的点突变与对甾醇脱甲基抑制剂的低敏感性相关

2016年，日本青森县发生了严重的苹果黑星病病菌（由黑斑病引起），这是由于真菌对甾醇去甲基化抑制剂（DMI）的敏感性降低所致。在这项研究中，羊毛甾醇14α去甲基化酶（CYP51A1）的基因苹果黑星菌从青森县株进行了分析。阿非同义核苷酸多态性在位置133（Y133F）引起氨基酸取代从酪氨酸至苯丙氨酸CYP51A1密切一起DMIS低灵敏度相关联。这是首次报道CYP51A1中的点突变与对V. inaequalis中DMI的低敏感性相关。