Intensive and semi-intensive aquaculture systems typically produce high loads of nutrients in their discharge, potentially leading to the development and persistence of harmful algal blooms (HABs). Algicidal bacteria, which inhibit algal growth or kill algal cells, offer a promising biocontrol strategy for reducing HAB. The strain JZBC1, which displays strong algicidal activity against the HAB species Scrippsiella trochoidea, was previously isolated from a dinoflagellate bloom area and identified as Bacillus cereus. In this study, strain JZBC1 was fermented in 2 L and 50 L systems, and then, algicidal properties of JZBC1 fermentation products on the harmful dinoflagellate species S. trochoidea, Prorocentrum micans, and Peridinium umbonatum were examined. Bacterial concentrations of strain JZBC1 reached 6.23 × 10⁹ and 4.47 × 10⁹ CFU/mL in 2 L and 50 L cultures, respectively, and thus effectively expanded during fermentation. Strain JZBC1 showed substantial algicidal activities and a wide algicidal range toward harmful dinoflagellates. Vegetative JZBC1 cells exhibited the best inhibitory and algicidal effects in 1 day. Strain JZBC1 can be formulated into a powder, and the spores can be activated before field application to increase their efficacy. These findings will facilitate large-scale fermentation production and application of Bacillus strain JZBC1 for controlling dinoflagellate blooms in aquaculture ponds.

集约化和半集约化水产养殖系统通常在其排放物中产生大量养分，有可能导致有害藻华（HAB）的产生和持续存在。抑制藻类生长或杀死藻类细胞的杀藻细菌为减少HAB提供了有希望的生物防治策略。菌株JZBC1表现出强大的抗HAB物种拟南芥（Scrippsiella trochoidea）杀藻活性，先前已从重鞭毛藻开花区分离出来，鉴定为蜡状芽孢杆菌。在这项研究中，应变JZBC1被发酵在2L和50升系统，然后，在有害甲藻物种的JZBC1发酵产物杀藻性能S.藻，海洋原甲藻，并检查了伞形idi。 在2 L和50 L培养物中，菌株JZBC1的细菌浓度分别达到6.23×10 ⁹和4.47×10 ⁹ CFU / mL，因此在发酵过程中有效扩展。菌株JZBC1对有害的鞭毛藻类显示出大量的杀藻活性和广泛的杀藻范围。营养JZBC1细胞在1天内表现出最佳的抑制和杀藻作用。可以将JZBC1菌株配制成粉末，并可以在田间施用之前激活孢子以提高其效力。这些发现将有助于大规模发酵生产和应用芽孢杆菌JZBC1菌株来控制水产养殖池塘中鞭毛藻的繁殖。