Compared to green fluorescent protein (GFP) based biosensors, red fluorescent protein (RFP) based biosensors are inherently advantageous because of reduced phototoxicity, decreased autofluorescence, and enhanced tissue penetration. However, there is a limited choice of RFP-based biosensors and development of each biosensor requires significant effort. Herein, we describe a general and convenient method which uses the genetically encoded amino acid, 3-aminotyrosine (aY), to convert GFPs and GFP-based biosensors into red.

中文翻译：

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基于绿色荧光蛋白的生物传感器红移的一般策略

与基于绿色荧光蛋白（GFP）的生物传感器相比，基于红色荧光蛋白（RFP）的生物传感器具有内在的优势，因为其光毒性降低，自发荧光降低以及组织穿透力增强。但是，基于RFP的生物传感器的选择有限，每种生物传感器的开发都需要大量的精力。在这里，我们描述了一种通用且方便的方法，该方法使用遗传编码的氨基酸3-氨基酪氨酸（aY）将GFP和基于GFP的生物传感器转换为红色。