The study of pulmonary samples from individuals who have died as a direct result of COVID-19 infection is vital to our understanding of the pathogenesis of this disease. Histopathologic studies of lung tissue from autopsy of patients with COVID-19 specific mortality are only just emerging. All existing reports have relied on traditional 2-dimensional slide-based histological methods for specimen preparation. However, emerging methods for high-resolution, massively multiscale imaging of tissue microstructure using fluorescence labeling and tissue clearing methods enable the acquisition of tissue histology in 3-dimensions, that could open new insights into the nature of SARS-Cov-2 infection and COVID-19 disease processes. In this article, we present the first 3-dimensional images of lung autopsy tissues taken from a COVID-19 patient, including 3D "virtual histology" of cubic-millimeter volumes of the diseased lung, providing unique insights into disease processes contributing to mortality that could inform frontline treatment decisions.

中文翻译：

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使用高分辨率透明组织显微镜对COVID-19患肺的多尺度三维病理学发现

对直接因COVID-19感染而死亡的个体进行肺样本研究的研究对于我们了解这种疾病的发病机理至关重要。仅对COVID-19特异性死亡患者进行尸检而对肺组织进行的组织病理学研究刚刚兴起。现有的所有报告都依靠传统的基于二维幻灯片的组织学方法进行标本制备。但是，新兴的使用荧光标记和组织清除方法对组织微观结构进行高分辨率，大规模多尺度成像的方法，使得能够以3维方式获取组织组织学信息，这可以为SARS-Cov-2感染和COVID的性质开辟新的见解。 -19病程。在本文中，我们展示了从COVID-19患者那里采集的肺解剖组织的第一个3维图像，