ABSTRACT Trim45 is one of the RING (really interesting new gene) finger containing E3 ligase, which belongs to TRIM (Tripartite motif) protein family. Its molecular biological functions have been well characterized but not in light of developmental aspects. Here, we are reporting its expression patterns and developmental functions in zebrafish embryos. First, maternal transcripts of trim45 were found at one cell stage while its zygotic messages appeared at 30% epiboly. trim45 transcripts were restricted to the optical tectum, hypothalamus, hindbrain, and pharyngeal endoderm at 24 hpf (hour post-fertilization), and further to the retinal ganglion cell layer and cranial ganglion at 36 hpf. Second, ectopic expression of trim45 by injecting its mRNAs into embryos at one cell stage caused significant expansion of the diencephalon and eye fields at 24 hpf. In contrast, knock-down of trim45 with anti-sense trim45 morpholinos reduced the size of the two tissues at 24 hpf. Finally, the spatial distribution of the transcripts from olig2 and rx1/rx3, markers for the midbrain and eye respectively, were significantly decreased in the thalamus and eye fields respectively at 24 hpf. Based upon these observations, we proposed possible roles of Trim45 in the development of the diencephalon and eye in zebrafish embryos.

中文翻译：

---

Trim45 对斑马鱼胚胎间脑和眼睛的发育至关重要

摘要 Trim45 是含有 E3 连接酶的 RING（真正有趣的新基因）指之一，属于 TRIM（三方基序）蛋白家族。其分子生物学功能已被很好地表征，但未在发育方面进行。在这里，我们报告了它在斑马鱼胚胎中的表达模式和发育功能。首先，trim45 的母体转录本是在一个细胞阶段发现的，而其合子信息出现在 30% 的外包。在 24 hpf（受精后小时），trim45 转录物仅限于视顶盖、下丘脑、后脑和咽内胚层，并在 36 hpf 时仅限于视网膜神经节细胞层和颅神经节。第二，通过在一个细胞阶段将其 mRNA 注射到胚胎中，trim45 的异位表达导致间脑和眼野在 24 hpf 时显着扩大。相比之下，用反义trim45 morpholinos 击倒trim45 可在24 hpf 时减少两种组织的大小。最后，来自 olig2 和 rx1/rx3 的转录本的空间分布，分别是中脑和眼睛的标记，在 24 hpf 时分别在丘脑和眼场中显着降低。基于这些观察，我们提出了 Trim45 在斑马鱼胚胎间脑和眼睛发育中的可能作用。在 24 hpf 时，中脑和眼睛的标记分别在丘脑和眼野中显着降低。基于这些观察，我们提出了 Trim45 在斑马鱼胚胎间脑和眼睛发育中的可能作用。在 24 hpf 时，中脑和眼睛的标记分别在丘脑和眼野中显着降低。基于这些观察，我们提出了 Trim45 在斑马鱼胚胎间脑和眼睛发育中的可能作用。