ABSTRACT Grapevine trunk diseases (GTDs), caused by a complex of fungi, are a global threat to vineyard longevity. Identifying the fungi in each growing region is crucial for interpreting symptoms and understanding disease progression. Despite considerable research, the spectrum of fungi involved in GTDs in New Zealand remains uncertain and species identities within key taxonomic groups require validation with molecular sequences. We provide a synopsis of fungi from known pathogenic GTD taxa that have been identified in culture-dependent studies in New Zealand, especially those accessible through culture collections such as the International Collection of Microorganisms from Plants (ICMP). Results from a 2007–2010 countrywide vineyard survey for GTD fungi are presented, plus new isolates and associated DNA sequences from a 2017 survey of Marlborough and Hawke’s Bay vineyards and from institutional microbial collections. These new records clarify that authentic Eutypa lata and Phaeomoniella chlamydospora fungi, which are widely studied GTD pathogens, are present and probably common in New Zealand. First records of Inonotus, Diatrype, Sporocadus and Phaeoacremonium species are presented from New Zealand vineyards. The DNA sequences presented from GTD pathogens will facilitate the use of molecular detection technologies for measuring pathogen distributions in New Zealand vineyards and for biosecurity detections.

中文翻译：

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在新西兰分离出与葡萄树干病相关的病原真菌

摘要 由真菌复合体引起的葡萄树干病 (GTD) 是对葡萄园寿命的全球性威胁。识别每个生长区域的真菌对于解释症状和了解疾病进展至关重要。尽管进行了大量研究，但新西兰参与 GTD 的真菌谱仍然不确定，关键分类群中的物种身份需要通过分子序列进行验证。我们提供了在新西兰依赖培养的研究中已确定的已知致病性 GTD 分类群的真菌概要，尤其是那些可通过培养物保藏中心获得的真菌，例如国际植物微生物保藏中心 (ICMP)。展示了 2007-2010 年全国葡萄园 GTD 真菌调查的结果，加上 2017 年对马尔堡和霍克斯湾葡萄园的调查以及机构微生物收集的新分离株和相关 DNA 序列。这些新记录阐明了真正的 Eutypa lata 和 Phaeomoniella chlamydospora 真菌，它们是被广泛研究的 GTD 病原体，它们在新西兰存在并且可能很常见。Inonotus、Diatrype、Sporocadus 和 Phaeoacremonium 物种的第一批记录来自新西兰的葡萄园。来自 GTD 病原体的 DNA 序列将有助于使用分子检测技术来测量新西兰葡萄园的病原体分布和生物安全检测。存在并且可能在新西兰很常见。Inonotus、Diatrype、Sporocadus 和 Phaeoacremonium 物种的第一批记录来自新西兰的葡萄园。来自 GTD 病原体的 DNA 序列将有助于使用分子检测技术来测量新西兰葡萄园的病原体分布和生物安全检测。存在并且可能在新西兰很常见。Inonotus、Diatrype、Sporocadus 和 Phaeoacremonium 物种的第一批记录来自新西兰的葡萄园。来自 GTD 病原体的 DNA 序列将有助于使用分子检测技术来测量新西兰葡萄园的病原体分布和生物安全检测。