The monoclonal antibody (mAb) CO17‐1A specifically binds to the tumor‐associated cell surface glycoprotein GA733 in colorectal cancer cells. Thus, mAb CO17‐1A has the potential to act as an immune therapeutic protein against colorectal cancer. Recently, it was shown that the baculovirus insect cell expression system produces anti‐colorectal cancer mAb CO17‐1A. In this study, the colorectal cancer antibody mAb CO17‐1A fused to the endoplasmic reticulum (ER) retention signal sequence (KDEL), and the (mAb CO17‐1AK) was expressed in Spodoptera frugiperda Sf9 insect cells. The yield, cell cytotoxicity, and in vitro anti‐tumor activity of mAb CO17‐1AK were verified. Western blotting was performed to confirm that both heavy and light chains of mAb CO17‐1A were expressed in Sf9 insect cells. The insect‐derived mAb (mAbI) CO17‐1A was purified using a protein G affinity column. An in vitro wound healing assay was conducted to determine the inhibition activity of mAb CO17‐1A during tumor cell migration, showing that mAbI CO17‐1AK was effective as mammalian‐derived mAb CO17‐1A (mAbM CO17‐1A). These results suggest that the insect cell expression system can produce and properly assemble mAbs that inhibit tumor cell migration.

中文翻译：

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体外伤口愈合：昆虫源性 mAb CO17-1A 在人结直肠癌细胞迁移中的抑制活性

单克隆抗体 (mAb) CO17-1A 特异性结合结直肠癌细胞中的肿瘤相关细胞表面糖蛋白 GA733。因此，mAb CO17-1A 有可能作为一种针对结直肠癌的免疫治疗蛋白。最近，表明杆状病毒昆虫细胞表达系统产生抗结直肠癌 mAb CO17-1A。在本研究中，结直肠癌抗体 mAb CO17-1A 与内质网 (ER) 滞留信号序列 (KDEL) 融合，并且 (mAb CO17-1AK) 在草地夜蛾 Sf9 昆虫细胞中表达。验证了 mAb CO17-1AK 的产量、细胞毒性和体外抗肿瘤活性。进行蛋白质印迹以确认 mAb CO17-1A 的重链和轻链均在 Sf9 昆虫细胞中表达。使用蛋白 G 亲和柱纯化昆虫衍生的 mAb (mAbI) CO17-1A。进行了体外伤口愈合试验以确定 mAb CO17-1A 在肿瘤细胞迁移过程中的抑制活性，表明 mAbI CO17-1AK 作为哺乳动物衍生的 mAb CO17-1A (mAbM CO17-1A) 是有效的。这些结果表明昆虫细胞表达系统可以产生并正确组装抑制肿瘤细胞迁移的单克隆抗体。