Combined effect of substerilizing doses of gamma radiation (40 and 100 Gy) and different concentrations of the entomopathogenic nematode, Steinernema carpocapsae BA2 (20 and 40 IJs/ml) on the hemocyte count of the greater wax moth, Galleria mellonella (L.) larvae was studied. Eight types of hemocytes were described in the hemolymph of the normal larvae of G. mellonella. Prohemocytes were the predominant type, while the cystocytes were the rare ones. Morphological malformations and changes in the number of each hemocyte type were observed in F1 larvae (of irradiated male parent pupae with 40 or 100 Gy) or larvae treated with different concentrations of the S. carpocapsae BA2 (normal or F1 larvae); these alterations were increased by increasing the radiation dose or the nematode concentration that led to increase the susceptibility of the larvae to the nematode. Therefore, it could be concluded that integration of entomopathogenic nematodes and gamma radiation may serve as integrated control program for G. mellonella.

中文翻译：

---

昆虫病原线虫，Steinernema carpocapsae和γ射线对大蜡蛾幼虫Galleria mellonella（L.）幼虫协同作用的血细胞研究

亚灭菌剂量的γ射线（40 Gy和100 Gy）和不同浓度的昆虫病原线虫，Steinernema carpocapsae BA2（20 IJs / ml和40 IJs / ml）对较大蜡蛾幼虫血细胞计数的综合影响被研究了。在G. mellonella正常幼虫的血淋巴中描述了八种类型的血细胞。前血细胞是主要类型，而膀胱细胞是稀有类型。在F1幼虫（用40或100 Gy照射的雄性亲本p）或用不同浓度的S. carpocapsae BA2（正常或F1幼虫）处理过的幼虫中，观察到形态学畸形和每种血细胞类型数目的变化；这些改变通过增加辐射剂量或线虫浓度而增加，从而导致幼虫对线虫的敏感性增加。因此，可以得出结论，昆虫病原线虫和伽马射线的整合可以作为梅毒线虫的综合控制程序。