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Efficacy of entomopathogenic nematodes against the cabbage butterfly (Pieris brassicae (L.) (Lepidoptera: Pieridae) infesting cabbage under field conditions
Egyptian Journal of Biological Pest Control ( IF 2.4 ) Pub Date : 2020-04-09 , DOI: 10.1186/s41938-020-00243-y
Tarique Hassan Askary , Mohammad Jamal Ahmad

Experiments were conducted to test the pathogenicity of entomopathogenic nematode, Heterorhabditis pakistanensis alone and in combination with the chemical insecticides, viz., Dichlorvos and Quinalphos and Neem product, viz., Azadirachtin against the cabbage butterfly, Pieris brassicae (L.) (Lepidoptera: Pieridae) infesting cabbage plants under laboratory and field conditions. In laboratory test, inoculum level of 200 infective juveniles (IJs) was most effective causing mortality to both 3rd and 4th instars larvae at 48 h. Nematode multiplication rate in insect cadaver was inversely proportional to the size of initial inoculum level of IJs, whereas IJs yield was directly proportional to the size of larva. The 4th instar larva resulted in a highest yield of IJs, followed by 3rd, 2nd, and 1st instars larvae at all the inoculum levels used in the study. IJs were found compatible with the chemical insecticides and Neem product at lower than half of the optimum single dose. The cumulative mean survival percentage of IJs recorded the highest (77.5%) in the combination of 100 IJs + Azadirachtin (0.25%) and lowest (70.6%) in 100 IJs + Dichlorvos 76 EC (0.05%). Under field conditions, H. pakistanensis (3.0 lakh IJs/m2) + Dichlorvos 76 EC (0.025%) resulted to the highest larval mortality (79.65%), followed by H. pakistanensis (3.0 lakh IJs/m2) + Azadirachtin (0.25%), where it was 76.22%. The larval mortality was the least (61.16%) in case of H. pakistanensis (3.0 lakh IJs/m2) when applied alone. H. pakistanensis + Dichlorvos resulted to the highest increase (31.42%) in cabbage yield over untreated control.

中文翻译:

病原性线虫对田间条件下侵染白菜的白菜蝴蝶(菜青虫(鳞翅目:菜青虫))的功效

进行了实验以测试昆虫病原线虫,巴基斯坦异种杆菌和与化学杀虫剂(即敌敌畏,Quinalphos和Neem产品,即印za素)联合使用对白菜蝶,菜青虫(Pieris brasicae(L.))的致病性(鳞翅目: Pieridae)在实验室和田间条件下侵染白菜植物。在实验室测试中,接种物水平为200的感染少年(IJs)最有效,可导致48龄三龄和四龄幼虫死亡。昆虫尸体中线虫的繁殖率与IJs初始接种量的大小成反比,而IJs的产量与幼虫的大小成正比。第四龄幼虫的IJ产量最高,其次是第三,第二,研究中使用的所有接种物水平的第一龄幼虫。发现IJ与化学杀虫剂和Neem产品相容,且低于最佳单剂量的一半。在100支IJs +印za素(0.25%)和100支IJs + Dichlorvos 76 EC(0.05%)的组合中,IJs的累积平均存活百分比最高(77.5%)。在田间条件下,巴基斯坦H.(30万IJs / m2)+敌敌畏76 EC(0.025%)导致幼虫死亡率最高(79.65%),其次是巴基斯坦H.(30万IJs / m2)+印za素(0.25%) ),占76.22%。单独施用时,巴基斯坦血吸虫的幼虫死亡率最低(61.16%)(30万IJs / m2)。与未经处理的对照相比,H。pakistanensis + Dichlorvos导致白菜产量最高增幅(31.42%)。发现IJ与化学杀虫剂和Neem产品相容,且低于最佳单剂量的一半。在100支IJs +印za素(0.25%)和100支IJs + Dichlorvos 76 EC(0.05%)的组合中,IJs的累积平均存活百分比最高(77.5%)。在田间条件下,巴基斯坦H.(30万IJs / m2)+敌敌畏76 EC(0.025%)导致幼虫死亡率最高(79.65%),其次是巴基斯坦H.(30万IJs / m2)+印za素(0.25%) ),占76.22%。单独施用时,巴基斯坦血吸虫的幼虫死亡率最低(61.16%)(30万IJs / m2)。与未经处理的对照相比,H。pakistanensis + Dichlorvos导致白菜产量最高增幅(31.42%)。发现IJ与化学杀虫剂和Neem产品相容,且低于最佳单剂量的一半。在100支IJs +印za素(0.25%)和100支IJs + Dichlorvos 76 EC(0.05%)的组合中,IJs的累积平均存活百分比最高(77.5%)。在田间条件下,巴基斯坦H.(30万IJs / m2)+敌敌畏76 EC(0.025%)导致幼虫死亡率最高(79.65%),其次是巴基斯坦H.(30万IJs / m2)+印za素(0.25%) ),占76.22%。单独施用时,H。pakistanensis(30万IJs / m2)的幼虫死亡率最低(61.16%)。与未经处理的对照相比,H。pakistanensis + Dichlorvos导致白菜产量最高增幅(31.42%)。在100支IJs +印za素(0.25%)和100支IJs + Dichlorvos 76 EC(0.05%)的组合中,IJs的累积平均存活百分比最高(77.5%)。在田间条件下,巴基斯坦H.(30万IJs / m2)+敌敌畏76 EC(0.025%)导致幼虫死亡率最高(79.65%),其次是巴基斯坦H.(30万IJs / m2)+印za素(0.25%) ),占76.22%。单独施用时,H。pakistanensis(30万IJs / m2)的幼虫死亡率最低(61.16%)。与未经处理的对照相比,H。pakistanensis + Dichlorvos导致白菜产量最高增幅(31.42%)。在100支IJs +印za素(0.25%)和100支IJs + Dichlorvos 76 EC(0.05%)的组合中,IJs的累积平均存活百分比最高(77.5%)。在田间条件下,巴基斯坦H.(30万IJs / m2)+敌敌畏76 EC(0.025%)导致幼虫死亡率最高(79.65%),其次是巴基斯坦H.(30万IJs / m2)+印za素(0.25%) ),占76.22%。单独施用时,H。pakistanensis(30万IJs / m2)的幼虫死亡率最低(61.16%)。与未经处理的对照相比,H。pakistanensis + Dichlorvos导致白菜产量最高增幅(31.42%)。巴基斯坦(3.0十亿IJs / m2)+敌敌畏76 EC(0.025%)导致幼虫死亡率最高(79.65%),其次是巴基斯坦嗜血杆菌(3.0十万IJs / m2)+印za素(0.25%),为76.22 %。单独施用时,H。pakistanensis(30万IJs / m2)的幼虫死亡率最低(61.16%)。与未经处理的对照相比,H。pakistanensis + Dichlorvos导致白菜产量最高增幅(31.42%)。巴基斯坦(3.0十亿IJs / m2)+敌敌畏76 EC(0.025%)导致幼虫死亡率最高(79.65%),其次是巴基斯坦嗜血杆菌(3.0十万IJs / m2)+印za素(0.25%),为76.22 %。单独施用时,H。pakistanensis(30万IJs / m2)的幼虫死亡率最低(61.16%)。与未经处理的对照相比,H。pakistanensis + Dichlorvos导致白菜产量最高增幅(31.42%)。
更新日期:2020-04-09
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