Maize (Zea mays L.) is one of the most economic crops in Egypt. Production of amylase from the waste of maize is the most economic and cheap renewable and most abundant raw materials present in environment. Biosynthesis of Cu-chitosan nanoparticles for amylase production by co-culturing between Trichoderma harzianum and Pseudomonas fluorescens at different ratios compared to free conditions was the main purpose of this study. The optimum ratio 8:2, recorded between P. fluorescens: T. harzianum, was the most promising for production of amylase produce 22.47 and 28.60 U/ml for free and nano, respectively. The UV visible spectral analysis Cu-chitosan NPs was 220 nm, while the mean diameter, using transmission electron microscopy was 0.5 μm. Application of fermented maize wastes by co-cultivation of P. fluorescence and T. harzianum, as a grain dressing before sowing declared the reduction in both root and foliar diseases during the maize growing season, starting from germination up to 70 days of its vegetative growth under field conditions. A promising approach is the creation and use of environmentally safe products, whose protective effect is based on the induction of hydrolase inhibitors in plants.

中文翻译：

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使用哈茨木霉菌和荧光假单胞菌共培养物发酵的玉米废料作为田间条件下的玉米病

玉米（Zea mays L.）是埃及最经济的作物之一。从玉米废料生产淀粉酶是环境中最经济，最便宜的可再生和最丰富的原材料。本研究的主要目的是通过与不同条件下的不同比例的哈茨木霉菌和荧光假单胞菌共培养生物合成Cu-壳聚糖纳米粒生产淀粉酶。在荧光假单胞菌与哈茨木霉之间记录的最佳比例为8：2，对于游离和纳米淀粉酶的产量分别为22.47和28.60 U / ml，是最有希望的。紫外可见光谱分析Cu-壳聚糖NPs为220 nm，而使用透射电子显微镜的平均直径为0.5μm。通过荧光和哈茨木霉的共培养应用发酵的玉米废料，玉米播种前先进行谷物拌种，以宣告玉米生长季节根部和叶面疾病的减少，从发芽开始到田间条件下其营养生长长达70天。一种有前途的方法是创建和使用对环境无害的产品，其保护作用基于植物中水解酶抑制剂的诱导。